Inheritance of inter-simple-sequence-repeat polymorphisms and linkage with a fusarium wilt resistance gene in chickpea

• Published in: Theoretical and applied genetics Vol. 96; no. 3/4; pp. 348 - 353
• Main Authors: Ratnaparkhe, M.B, Santra, D.K, Tullu, A, Muehlbauer, F.J
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.03.1998; Berlin Springer Nature B.V
• Subjects: Anopheles; Biological and medical sciences; chromosome mapping; Cicer; Cicer arietinum; cicer reticulatum; Classical genetics, quantitative genetics, hybrids; crossing; Fundamental and applied biological sciences. Psychology; Fusarium; Fusarium oxysporum f. sp. ciceris; Genes; Genetic aspects; genetic markers; genetic polymorphism; Genetics; Genetics of eukaryotes. Biological and molecular evolution; Genomics; inbred lines; inheritance (genetics); interspecific hybridization; linkage (genetics); linkage groups; loci; Pteridophyta, spermatophyta; random amplified polymorphic DNA technique; repetitive sequences; segregation; Vegetals; wild relatives; wilts; Genetic mapping; Fusarium oxysporum; Linkage; Genetic marker; Plant pathogen; Chromosome; Fungus resistance; Fungi; Grain legume; Leguminosae; Gene; Cicer arietinum; Dicotyledones; Microsatellite DNA; Angiospermae; Spermatophyta; Fungi Imperfecti; Inheritance(genetics); Thallophyta; Polymorphism
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220050747

The inheritance of an inter-simple-sequence-repeat (ISSR) polymorphism was studied in a cross of cultivated chickpea (Cicer arietinum L.) and a closely related wild species (C. reticulatum Lad.) using primers that anneal to a simple repeat of various lengths, sequences and non-repetitive motifs. Dinucleotides were the majority of those tested, and provided all of the useful banding patterns. The ISSR loci showed virtually complete agreement with expected Mendelian ratios. Twenty two primers were used for analysis and yielded a total of 31 segregating loci. Primers based on (GA)n repeats were the most abundant while primers with a (TG)n repeat gave the largest number of polymorphic loci. Nucleotides at the 5' and 3' end of the primers played an important role in detecting polymorphism. All the markers showed dominance. We found an ISSR marker linked to the gene for resistance to fusarium wilt race 4. The marker concerned, UBC-855(500), was found to be linked in repulsion with the fusarium wilt resistance gene at a distance of 5.2 cM. It co-segregated with CS-27(700), a RAPD marker previously shown to be linked to the gene for resistance to fusarium wilt race 1, and was mapped to linkage group 6 of the Cicer genome. This indicated that genes for resistance to fusarium wilt races 1 and 4 are closely linked. The marker UBC-855(500) is located 0.6 cM from CS-27(700) and is present on the same side of the wilt resistance gene. To our knowledge this is the first report of the utility of an ISSR marker in gene tagging. These markers may provide valuable information for the development of sequence-tagged microsatellite sites (STMS) at a desired locus.