Effect of Hyaluronidase Immobilized Using Electron-Beam Synthesis Nanotechnology on Sensitivity of Progenitor Cells to Regulatory Factors

• Published in: Bulletin of experimental biology and medicine Vol. 151; no. 1; pp. 150 - 153
• Main Authors: Dygai, A. M., Zyuz’kov, G. N., Zhdanov, V. V., Udut, E. V., Miroshnichenko, L. A., Khrichkova, T. Yu, Simanina, E. V., Stavrova, L. A., Chaikovskiy, A. V., Markova, T. S., Minakova, M. Yu, Gol’dberg, V. E., Artamonov, A. V., Bekarev, A. A., Madonov, P. G., Kinsht, D. N., Gurto, R. V.
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.05.2011; Springer; Springer Nature B.V
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; Bone Marrow Cells - cytology; Bone Marrow Cells - drug effects; Cell Biology; Colony-Forming Units Assay; Cytokines; Enzymes, Immobilized - chemistry; Enzymes, Immobilized - pharmacology; Erythropoietin; Erythropoietin - pharmacology; Fibroblast growth factors; Fibroblast Growth Factors - pharmacology; Granulocyte Colony-Stimulating Factor - pharmacology; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - drug effects; Hyaluronoglucosaminidase - chemistry; Hyaluronoglucosaminidase - pharmacology; Insulin - metabolism; Insulin - pharmacology; Internal Medicine; Laboratory Medicine; Liver - cytology; Liver - drug effects; Male; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - drug effects; Mice; Mice, Inbred CBA; Nanotechnology; Pathology; Stem Cell Factor - pharmacology; Tissue Culture Techniques; Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine); nanotechnology; progenitor cells; cytokines; drugs; immobilized hyaluronidase
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-011-1277-0

In vitro experiments demonstrated increased colony-forming capacity of erythroid, granulomonocytic, and mesenchymal progenitors of the bone marrow and parenchymal progenitor elements of the liver after treatment with immobilized hyaluronidase. Increased sensitivity of these progenitor cells to erythropoietin, granulocyte colony-stimulating factor, fibroblast growth factor, and stem cell factor, respectively, was demonstrated. Immobilized hyaluronidase enhanced the formation of tissue-specific hepatic CFU against the background of reduced yield of stromal precursors in liver tissue culture containing insulin.