Interrogating lncRNA functions via CRISPR/Cas systems

Long noncoding RNAs (lncRNAs) are an increasing focus of investigation due to their implications in diverse biological processes and disease. Nevertheless, the majority of lncRNAs are low in abundance and poorly conserved, posing challenges to functional studies. The CRISPR/Cas system, an innovative...

Full description

Saved in:
Bibliographic Details
Published inRNA biology Vol. 18; no. 12; pp. 2097 - 2106
Main Authors S. Zibitt, Meira, Hartford, Corrine Corrina R., Lal, Ashish
Format Journal Article
LanguageEnglish
Published United States Taylor & Francis 02.12.2021
Subjects
Animals
CRISPR-Cas Systems
crispr/cas9
Gene Knockdown Techniques - methods
genome editing
Humans
lincRNA
Lncrna
Review
RNA, Long Noncoding - genetics
genome editing
Lncrna
lincRNA
crispr/cas9
Online AccessGet full text

Cover

More Information
Summary:Long noncoding RNAs (lncRNAs) are an increasing focus of investigation due to their implications in diverse biological processes and disease. Nevertheless, the majority of lncRNAs are low in abundance and poorly conserved, posing challenges to functional studies. The CRISPR/Cas system, an innovative technology that has emerged over the last decade, can be utilized to further understand lncRNA function. The system targets specific DNA and/or RNA sequences via a guide RNA (gRNA) and Cas nuclease complex. We and others have utilized this technology in various applications such as lncRNA knockout, knockdown, overexpression, and imaging. In this review, we summarize how the CRISPR/Cas technology provides new tools to investigate the roles and therapeutic implications of lncRNAs.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
content type line 23
ObjectType-Review-1
Equal contribution
ISSN:1547-6286
1555-8584
DOI:10.1080/15476286.2021.1899500