STXBP1 forms amyloid-like aggregates in rat brain and demonstrates amyloid properties in bacterial expression system

Amyloids are the fibrillar protein aggregates with cross-β structure. Traditionally amyloids were associated with pathology, however, nowadays more data is emerging about functional amyloids playing essential roles in cellular processes. We conducted screening for functional amyloids in rat brain. O...

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Published inPrion Vol. 15; no. 1; pp. 29 - 36
Main Authors Chirinskaite, A.V., Siniukova, V.A., Velizhanina, M.E., Sopova, J.V., Belashova, T.A., Zadorsky, S.P.
Format Journal Article
LanguageEnglish
Published United States Taylor & Francis 01.01.2021
Subjects
amyloid
Amyloid - metabolism
Amyloidosis
Animals
brain
Brain - metabolism
functional amyloid
Munc18 Proteins - metabolism
Munc18-1
Proteomic screening
Rapid Communication
rat
Rats
Short Communicatios
SNARE
STXBP1
amyloid
rat
STXBP1
Proteomic screening
Munc18-1
brain
functional amyloid
SNARE
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Summary:Amyloids are the fibrillar protein aggregates with cross-β structure. Traditionally amyloids were associated with pathology, however, nowadays more data is emerging about functional amyloids playing essential roles in cellular processes. We conducted screening for functional amyloids in rat brain. One of the identified proteins was STXBP1 taking part in vesicular transport and neurotransmitter secretion. Using SDD-AGE and protein fractionation we found out that STXBP1 forms small detergent-insoluble aggregates in rat brain. With immunoprecipitation analysis and C-DAG system, we showed that STXBP1 forms amyloid-like fibrils. Thus, STXBP1 demonstrates amyloid properties in rat brain and in bacterial expression system.
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ISSN:1933-6896
1933-690X
DOI:10.1080/19336896.2021.1883980