Immobilization of Horseradish Peroxidase on Multi-Armed Magnetic Graphene Oxide Composite Improvement of Loading Amount and Catalytic Activity

In this study, a novel type of multi-armed polymer (poyltehylene glycol, PEG) magnetic graphene oxide (GO) composite (GO@Fe3O4@6arm-PEG-NH2) has been synthesized as a support for immobilization of horseradish peroxidase (HRP) for the first time. The loading amount of HRP was relatively high (186.34...

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Published inFood technology and biotechnology Vol. 57; no. 2; pp. 260 - 271
Main Authors Zhou, Zijuan, Wang, Yun, Han, Juan, Li, Chunmei, Zhang, Wenli, Ni, Liang, Rong, Junhui
Format Journal Article
LanguageEnglish
Published Zagreb Sveuciliste U Zagrebu 01.04.2019
Sveuciliste u Zagrebu, Prehramheno-Biotehnoloski Fakultet
University of Zagreb Faculty of Food Technology and Biotechnology
Subjects
Amino groups
Aqueous solutions
Catalytic activity
Catalytic oxidation
Comparative analysis
Enzymes
Glycols (Class of compounds)
Graphene
graphene oxide
Graphite
Horseradish peroxidase
Hydrochloric acid
Hydrogen peroxide
Immobilization
immobilized enzyme
Iron oxides
Magnetic fields
multi-armed polymer
Nanoparticles
Novels
Original Scientific Papers
Peroxidase
Phenols
Phenols (Class of compounds)
Polyethylene glycol
Polymer industry
Polymerization
Polymers
Proteins
Shelf life
Synergistic effect
China
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Summary:In this study, a novel type of multi-armed polymer (poyltehylene glycol, PEG) magnetic graphene oxide (GO) composite (GO@Fe3O4@6arm-PEG-NH2) has been synthesized as a support for immobilization of horseradish peroxidase (HRP) for the first time. The loading amount of HRP was relatively high (186.34 mg/g) due to the surface of carrier material containing a large amount of amino groups from 6arm-PEG-NH2, but degradation rate of phenols was also much higher (95.4 %), which is attributed to the synergistic effect between the free HRP (45.4 %) and the support material of GO@Fe3O4@6arm-PEG-NH2 (13.6 %). Compared with the free enzyme, thermal, storage and operational stability of the immobilized HRP improved. The immobilized HRP still retained over 68.1 % activity after being reused 8 times. These results suggest that the multi-armed magnetic composite has good application prospect for enzyme immobilization.
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ISSN:1330-9862
1334-2606
DOI:10.17113/ftb.57.02.19.5832