Direct effects of ethanol on neuronal differentiation: an in vitro analysis of viability and morphology

• Published in: Brain research bulletin Vol. 127; pp. 177 - 186
• Main Authors: Guadagnoli, T, Caltana, L, Vacotto, M, Gironacci, M.M, Brusco, A
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2016
• Subjects: Animals; Apoptosis - drug effects; Apoptosis - physiology; Blotting, Western; Cell Nucleus Shape - drug effects; Cell Survival - drug effects; Cell Survival - physiology; Cells, Cultured; Cerebral Cortex - drug effects; Cerebral Cortex - pathology; Cerebral Cortex - physiopathology; Cytoskeleton - drug effects; Cytoskeleton - metabolism; Cytoskeleton - pathology; DNA Breaks, Double-Stranded - drug effects; Ethanol; Ethanol - toxicity; Fluorescent Antibody Technique; In Situ Nick-End Labeling; Microscopy, Electron; Microscopy, Fluorescence; Microtubule-Associated Proteins - metabolism; Neurogenesis - drug effects; Neurogenesis - physiology; Neurology; Neuronal death; Neuronal morphology; Neurons - drug effects; Neurons - pathology; Neurons - physiology; Rats, Wistar; Synapse; Synapses - drug effects; Synapses - pathology; Synapses - physiology; Dulbecco’s modified Eagle’s medium; [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay; Caspase 9 active; EtOH; CNS; microtubules; MTT; ethanol; Caspase 3 active; DMEM; TUNEL; MAP2; EB; gliofibrillary acidic protein; PBS; Caspase 9a; Synaptophysin; SSC; microtubule-associated proteins 2; dUTP-mediated nicked end labeling; Caspase 3a; MT; Syn; synapse; AO; acridine orange; neuronal morphology; GFAP; saline sodium citrate buffer; Central Nervous System; PSD95; phosphate buffered saline; neuronal death; Postsynaptic density protein 95; ethidium bromide; Ethanol; Synapse; Neuronal death; Neuronal morphology
• ISSN: 0361-9230; 1873-2747
• DOI: 10.1016/j.brainresbull.2016.09.013

Highlights • EtOH induces neuronal death. • Cytoskeleton and organelles are disrupted by EtOH. • EtOH exposure alters synapse formation.