Competitive Exclusion Reduces Transmission and Excretion of Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Broilers

• Published in: Applied and environmental microbiology Vol. 83; no. 11
• Main Authors: Ceccarelli, Daniela, van Essen-Zandbergen, Alieda, Smid, Bregtje, Veldman, Kees T, Boender, Gert Jan, Fischer, Egil A J, Mevius, Dik J, van der Goot, Jeanet A
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.06.2017
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; beta-Lactamases - genetics; beta-Lactamases - metabolism; Broiler chicken; Chickens; Competitive exclusion; Enterobacteriaceae; ESBL; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - enzymology; Escherichia coli - genetics; Escherichia coli - isolation & purification; Escherichia coli Infections - microbiology; Escherichia coli Infections - transmission; Escherichia coli Infections - veterinary; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Excretion; Intervention; Netherlands; Poultry; Poultry Diseases - microbiology; Poultry Diseases - transmission; Public and Environmental Health Microbiology; Transmission; β-lactamases; β-lactamases; competitive exclusion; poultry; transmission; excretion; Escherichia coli; ESBL; intervention; broiler chicken
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.03439-16

Extended-spectrum β-lactamases (ESBLs) and plasmid-mediated AmpC β-lactamases (pAmpC) are enzymes able to hydrolyze a large variety of β-lactam antibiotics, including third-generation cephalosporins and monobactams. Broilers and broiler meat products can be highly contaminated with ESBL- and pAmpC-producing strains, also known as extended-spectrum cephalosporin (ESC)-resistant strains, and can be a source for human infections. As few data on interventions to reduce the presence of ESC-resistant in broilers are available, we used transmission experiments to examine the role of competitive exclusion (CE) on reducing transmission and excretion in broilers. A broiler model to study the transmission of ESC-resistant was set up. Day-old chickens were challenged with an ESBL-producing strain isolated from healthy broilers in the Netherlands. Challenged and not challenged chicks were housed together in pairs or in groups, and ESBL-producing transmission was monitored via selective culturing of cloacal swab specimens. We observed a statistically significant reduction in both the transmission and excretion of ESBL-producing in chicks treated with the probiotic flora before challenge compared to the transmission and excretion in untreated controls. In conclusion, our results support the use of competitive exclusion as an intervention strategy to control ESC-resistant in the field. Extended-spectrum β-lactamases (ESBLs) and plasmid-mediated AmpC β-lactamases are a primary cause of resistance to β-lactam antibiotics among members of the family in humans, animals, and the environment. Food-producing animals are not exempt from this, with a high prevalence being seen in broilers, and there is evidence pointing to a possible foodborne source for human contamination. We investigated the effect of administration of a commercial probiotic product as an intervention to reduce the amount of ESBL-producing in broilers. Our results showed a substantial reduction in the level of colonization of broiler intestines by ESBL-producing after administration of commercial probiotic product. The protective effect provided by these probiotics could be implemented on a larger scale in poultry production. Reductions in the levels of ESBL-producing in the food chain would considerably benefit public health.