Microemulsion-based organogels as matrices for lipase immobilization

• Published in: Biotechnology advances Vol. 28; no. 3; pp. 395 - 406
• Main Authors: Zoumpanioti, Maria, Stamatis, Haralambos, Xenakis, Aristotelis
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Inc 01.05.2010; Elsevier
• Subjects: Adsorption; Biocatalysis; Bioconversions. Hemisynthesis; Biological and medical sciences; Biotechnology; Derivatives; Emulsions - chemistry; Enantioselectivity; Enzyme Activation; Enzyme Stability; Enzymes; Enzymes, Immobilized - chemistry; Esterifications; Fundamental and applied biological sciences. Psychology; Gelatin; Gels - chemistry; General aspects; Hydroxy propyl methyl cellulose; Immobilization; Immobilization techniques; Lipase; Lipase - chemistry; Mathematical analysis; Matrices; Matrix methods; Methods. Procedures. Technologies; Microemulsions; Organic Chemicals - chemistry; Protein Binding; Biocatalysis; Enantioselectivity; Lipase; Esterifications; Gelatin; Hydroxy propyl methyl cellulose; Enzyme; Cellulose; Immobilization; Triacylglycerol lipase; Esterases; Esterification; Carboxylic ester hydrolases; Hydrolases
• ISSN: 0734-9750; 1873-1899; 1873-1899
• DOI: 10.1016/j.biotechadv.2010.02.004

Organogels based on water-in-oil microemulsions can be formed using various natural polymers such as gelatin, agar or cellulose derivatives. Enzymes entrapped in the water core of the microemulsion can keep their activity and enhance their stability within the gel matrix. The importance of the microemulsion based organogels (MBGs) leans on their numerous potential biotechnological applications. An important example is the use of various lipase microemulsion systems for hydrolytic or synthetic reactions. In this review, several MBGs are being evaluated as immobilization matrices for various enzymes. The main subject focuses on the parameters that affect the use of MBGs as media for bioorganic reactions using lipases as catalysts.