Some hydrolase activities from the tick Hyalomma lusitanicum Koch, 1844 (Ixodoidea: Ixodida)

• Published in: Parasite (Paris) Vol. 15; no. 4; pp. 589 - 593
• Main Authors: Giménez-Pardo, C., Martínez-Grueiro, M.M.
• Format: Journal Article
• Language: English
• Published: Paris EDP Sciences 01.12.2008; Princeps
• Subjects: Acetylcholinesterase - metabolism; Acetylcholinesterase - physiology; Animals; Arachnid Vectors - enzymology; Arachnid Vectors - parasitology; Arachnida; Biochemistry. Physiology. Immunology; Biological and medical sciences; caractérisation préliminaire; Cattle; Drug Resistance; Female; Fundamental and applied biological sciences. Psychology; Hyalomma lusitanicum; hydrolase; hydrolases; Hydrolases - metabolism; Hydrolases - physiology; Invertebrates; Ixodidae; Male; Medically important nuisances and vectors, pests of stored products and materials: population survey and control; Phosphoric Monoester Hydrolases - metabolism; Phosphoric Monoester Hydrolases - physiology; Physiology. Development; preliminary characterization; Theileria annulata; Theileriasis - transmission; Tick Infestations - prevention & control; Tick Infestations - veterinary; ticks; Ticks - enzymology; Ticks - parasitology; tique; Vaccines; Vectors. Intermediate hosts; Parasitiformes; Theileriosis; Protozoal disease; Enzyme; Parasite; Parasitosis; Infection; Arachnida; Acari; Enzymatic activity; Ixodida; Arthropoda; Hydrolases; Ectoparasite; Invertebrata; Vector; Ixodidae
• ISSN: 1252-607X; 1776-1042
• DOI: 10.1051/parasite/2008154589

In this work has been made a detection and preliminary characterization of some hydrolases in whole extracts from unfed adult males and females of Hyalomma lusitanicum, one of the vectors for Theileria annulata that causes Mediterranean theileriosis in cattle. We have elected as targets, proteases as enzymes implicated in the nutritional processes of ticks, esterases that are usually implicated in resistance to organophosphates and phosphatises often implicated in protein phosphorilation and control of ticks salivary gland. The biological role and physiological significance are discussed in terms of the possibility of use these enzymes as possible in future anti-tick vaccination or acaricide resistance. Ce travail a porté sur la détection et la caractérisation préliminaire de diverses hydrolases extraites de mâles et de femelles adultes et non gorgées de Hyalomma lusitanicum, l’un des vecteurs de Theileria annulata, agent responsable de la theilériose méditerranéenne des bovins. Nous avons ciblé les recherches sur les protéases (retrouvées dans le processus de digestion), les estérases (souvent impliquées dans la résistance aux organophosphorés) et les phosphatases (phosphorilation des protéines et contrôle de l’activité des glandes salivaires) des tiques. Le rôle biologique et l’importance physiologique de ces enzymes sont discutés en termes de possibilité d’utilisation dans la vaccination et la résistance des tiques aux acaricides.