Neurovascular coupling protects neurons against hypoxic injury via inhibition of potassium currents by generation of nitric oxide in direct neuron and endothelium cocultures

• Published in: Neuroscience Vol. 334; pp. 275 - 282
• Main Authors: Wu, Kun-Wei, Kou, Zeng-Wei, Mo, Jia-Lin, Deng, Xu-Xu, Sun, Feng-Yan
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 15.10.2016
• Subjects: Animals; brain microvascular endothelial cells; Cell Hypoxia - drug effects; Cell Hypoxia - physiology; Cell Survival - drug effects; Cell Survival - physiology; Cerebral Cortex - drug effects; Cerebral Cortex - metabolism; Cerebral Cortex - pathology; Coculture Techniques; Endothelium - drug effects; Endothelium - metabolism; Endothelium - pathology; Glucose - deficiency; Microvessels - drug effects; Microvessels - metabolism; Microvessels - pathology; Neurology; Neurons - drug effects; Neurons - metabolism; Neurons - pathology; Neuroprotection - drug effects; Neuroprotection - physiology; Neurovascular Coupling - drug effects; Neurovascular Coupling - physiology; neurovascular unit; nitric oxide; Nitric Oxide - metabolism; Nitric Oxide Synthase - antagonists & inhibitors; Nitric Oxide Synthase - metabolism; patch clamp; Patch-Clamp Techniques; Potassium - metabolism; potassium channel; Potassium Channels - metabolism; Rats, Sprague-Dawley; stroke; NO; GTP; ethylene glycol-bis-β-aminoethyl ether N,N,N′,N′-tetra acetic acid; K; Kv; NVU; brain microvascular endothelial cells; SNP; sodium nitroprusside; propidium iodide; TTX; oxygen and glucose deprivation; neurovascular unit; voltage-gated potassium currents; AC; nitric oxide; patch clamp; l-NAME; I A; BMECs; OGD; stroke; EGTA; nitro- l-arginine methylester; adenylate cyclase; I K; potassium ions; HEPES; adenosine triphosphate; outward delayed-rectifier potassium currents; PI; potassium channel; guanosine-5′-triphosphate; tetrodotoxin; ATP; N-2-hydroxyethylpiper-azine-N′-2-ethanesulfonic acid; transient outward potassium currents; IK; BMEC; IA
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2016.08.012

Highlights • BMEC inhibited the potassium currents of neurons in direct neuron-BMEC cocultures. • BMEC reduced the IA of cocultured neurons via production of NO. • BMEC protected cocultured neurons against hypoxic injury via reduction of neuronal IA.