Long non-coding RNA CCAT1 promotes cervical cancer cell proliferation and invasion by regulating the miR-181a-5p/MMP14 axis

• Published in: Cell cycle (Georgetown, Tex.) Vol. 18; no. 10; pp. 1110 - 1121
• Main Authors: Shen, Hui, Wang, Liming, Xiong, Jinfeng, Ren, Ci, Gao, Chun, Ding, Wencheng, Zhu, Da, Ma, Ding, Wang, Hui
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 19.05.2019
• Subjects: CCAT1; Cell Proliferation - genetics; Cervical cancer; Female; Humans; LncRNA; Matrix Metalloproteinase 14 - genetics; Matrix Metalloproteinase 14 - metabolism; microRNA; MicroRNAs - genetics; MicroRNAs - metabolism; Neoplasm Invasiveness - genetics; Research Paper; RNA, Long Noncoding - physiology; Up-Regulation; Uterine Cervical Neoplasms - genetics; Uterine Cervical Neoplasms - pathology; LncRNA; CCAT1; microRNA; Cervical cancer
• ISSN: 1538-4101; 1551-4005; 1551-4005
• DOI: 10.1080/15384101.2019.1609829

Cervical cancer is a serious threat to women's health and is the third most common malignancy in women worldwide. Recent studies indicate that the long non-coding RNA CCAT1 plays a role in the malignant behavior of many tumors. However, the role of CCAT1 in cervical cancer is still unknown. Our aim is to evaluate the expression and investigate the regulatory role and potential mechanism of CCAT1 in cervical cancer. CCAT1 expression was measured by qRT-PCR. In addition, CCK-8 assays, colony formation assays, qRT-PCR assays, Transwell assays and xenograft experiments were performed to determine the role of CCAT1 in the proliferation and invasion in cervical cancer cells. The expression of CCAT1 in the cervical cancer tissues was higher than in the adjacent normal tissues. Overexpressing CCAT1 promoted cervical cancer cell proliferation, colony formation, and invasion in vitro. Elevated CCAT1 suppressed miR-181a expression, which was accompanied by an increased expression of MMP14 and HB-EGF. In contrast, knocking down CCAT1 resulted in increased expression of miR-181a, along with decreased expression of MMP14 and HB-EGF. Thus, CCAT1 is a key oncogenic lncRNA associated with cervical cancer and plays a role in promoting cervical cancer cell proliferation and invasion by regulating the miR-181a-5p/MMP14 axis.