Elevated marrow inflammatory cells and osteoclasts in subchondral osteosclerosis in human knee osteoarthritis
ABSTRACT Subchondral osteosclerosis, characterized by an increase of hypomineralized bone material, is a pathological hallmark of osteoarthritis. The cellular components in the subchondral marrow compartment that participate in this aberrant bone remodeling process remain to be elucidated. This stud...
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Published in | Journal of orthopaedic research Vol. 34; no. 2; pp. 262 - 269 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Blackwell Publishing Ltd
01.02.2016
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Subjects | |
Online Access | Get full text |
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Summary: | ABSTRACT
Subchondral osteosclerosis, characterized by an increase of hypomineralized bone material, is a pathological hallmark of osteoarthritis. The cellular components in the subchondral marrow compartment that participate in this aberrant bone remodeling process remain to be elucidated. This study assessed the presence of marrow inflammatory cells and their relative abundance between nonsclerotic and sclerotic tissues in knee osteoarthritis. Bone samples from osteoarthritic knee tibial plateaus were stratified for histological analyses using computed tomography osteoabsorptiometry. Immunohistological analysis revealed the presence of CD20 (B‐lymphocyte) and CD68 (macrophage), but not CD3 (T‐lymphocyte) immunoreactive mononuclear cells in subchondral marrow tissues and their relative abundance was significantly increased in sclerotic compared with nonsclerotic bone samples. Multinucleated osteoclasts that stained positive for CD68 and tartrate‐resistant acid phosphatase, predominantly associated with CD34‐positive blood vessels and their abundance was strongly increased in sclerotic samples. Bone‐specific alkaline phosphatase activity in outgrowth osteoblasts was induced by conditioned medium from nonsclerotic, but not sclerotic, bone pieces. These results suggest that an interaction between bone‐resident cells and marrow inflammatory cells might play a role in aberrant bone remodeling leading to subchondral osteosclerosis. Elevated osteoclast activity in sclerotic bone suggests that bone formation and resorption activities are increased, yet uncoupled, in human knee osteoarthritis. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:262–269, 2016. |
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Bibliography: | istex:C94564CB101056DEF30CB0DB104F1F51CADE518D Gottfried and Julia Bangerter-Rhyner Foundation University of Basel Research Fund Abbott Rheumatology Grant ark:/67375/WNG-BNM0F2HS-1 ArticleID:JOR23009 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0736-0266 1554-527X |
DOI: | 10.1002/jor.23009 |