Ontogeny of the stomach in yellow catfish (Pelteobagrus fulvidraco): detection and quantifictation of pepsinogen and H+/K+-ATPase gene expression

• Published in: Journal of animal physiology and animal nutrition Vol. 97; no. 1; pp. 20 - 26
• Main Authors: Gao, C., Yang, R. B., Hu, W. B., Wang, J.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.02.2013; Wiley Subscription Services, Inc
• Subjects: Acidification; Aging; Animals; Aquaculture; Base Sequence; Catfishes - anatomy & histology; Catfishes - classification; Catfishes - growth & development; Commercial species; Data processing; Development; Gastric glands; Gene expression; Gene Expression Regulation, Developmental - physiology; Gene Expression Regulation, Enzymologic; H(+)-K(+)-Exchanging ATPase - genetics; H(+)-K(+)-Exchanging ATPase - metabolism; H+/K+-ATPase; Hydrogen; larvae; Ontogeny; Pelteobagrus fulvidraco; pepsinogen; Pepsinogen A - genetics; Pepsinogen A - metabolism; Polymerase chain reaction; real-time PCR; Stomach; Weaning
• ISSN: 0931-2439; 1439-0396
• DOI: 10.1111/j.1439-0396.2011.01238.x

Summary Yellow catfish (Pelteobagrus fulvidraco) is an important commercial species with high aquaculture potential in China. To better understand the process of digestive functioning of gastric gland development during the larval from 1 dph (day post‐hatching) to 30 dph, real‐time PCR was used to detect and quantify the pepsinogen and H+/K+‐ATPase gene expression in P. fulvidraco. These data were also compared with the adult situation. The results showed that the expression of pepsinogen and H+/K+‐ATPase genes in P. fulvidraco larvae both started at 1 dph, though the expression level was very low until 3 dph. The quantification of pepsinogen gene expression increased significantly from 4 to 8 dph, increased fluctuantly from 8 to 23 dph and rose sharply from 23 to 30 dph. In comparison with adult fish, there were no significant differences with larvae at 5 and 23 dph. However, data of 10 and 30 dph larvae were obviously higher than those of adult group. H+/K+‐ATPase gene expression increased linearly from 1 to 30 dph. However, it was significantly lower than that of adult. The results show that P. fulvidraco larvae have an earlier functional stomach, though the function of the stomach is still not perfect. There is a gradual acidification environment within the stomach during the P. fulvidraco larvae development. Based on these results, we suggest that the weaning time for P. fulvidraco larvae would be much better after 23 dph.