Measurement of tryptophan hydroxylase activity in rat pineal glands and pinealocytes using an HPLC assay with electrochemical detection

A method for measuring tryptophan hydroxylase activity by assaying the product 5-HTP using high-performance liquid chromatography (HPLC) with electrochemical detection is described. A nocturnal elevation (80%) in rat pineal gland tryptophan hydroxylase activity was detected. Experiments on isolated...

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Bibliographic Details
Published inJournal of pineal research Vol. 6; no. 3; p. 285
Main Authors Sugden, D, Grady, Jr, R, Mefford, I N
Format Journal Article
LanguageEnglish
Published England 01.01.1989
Subjects
5-Hydroxytryptophan - analysis
Animals
Chromatography, High Pressure Liquid - methods
Electrochemistry
Female
Pineal Gland - cytology
Pineal Gland - enzymology
Rats
Rats, Inbred Strains
Tryptophan Hydroxylase - metabolism
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Summary:A method for measuring tryptophan hydroxylase activity by assaying the product 5-HTP using high-performance liquid chromatography (HPLC) with electrochemical detection is described. A nocturnal elevation (80%) in rat pineal gland tryptophan hydroxylase activity was detected. Experiments on isolated rat pinealocytes with the protein synthesis inhibitor cycloheximide indicate that tryptophan hydroxylase turns over rapidly in these cells. This method will be valuable in studies of the adrenergic mechanisms regulating pineal tryptophan hydroxylase activity.
ISSN:0742-3098
DOI:10.1111/j.1600-079X.1989.tb00424.x