Cyclophilin Participates in Responding to Stress Situations in Porphyra haitanensis (Bangiales, Rhodophyta)

Porphyra haitanensis (T. J. Chang & B. F. Zheng) is an important economic alga found off the southern coast of China. It has evolved a strong tolerance against stress, which is an important survival characteristic. Cyclophilin has been shown to be involved in the stress response of plants and al...

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Published inJournal of phycology Vol. 49; no. 1; pp. 194 - 201
Main Authors Jia, Zhaojun, Niu, Jianfeng, Huan, Li, Wu, Xiaojie, Wang, Guangce, Hou, Zhaojun
Format Journal Article
LanguageEnglish
Published United States Blackwell Publishing Ltd 01.02.2013
Wiley Subscription Services, Inc
Subjects
Algae
Bangiales
cyclophilin
multifactorial stress
Porphyra
Porphyra haitanensis
real-time PCR quantification
Rhodophyta
Stress
stress responding
China, People's Rep
stress responding
cyclophilin
real-time PCR quantification
Porphyra haitanensis
multifactorial stress
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Summary:Porphyra haitanensis (T. J. Chang & B. F. Zheng) is an important economic alga found off the southern coast of China. It has evolved a strong tolerance against stress, which is an important survival characteristic. Cyclophilin has been shown to be involved in the stress response of plants and algae. To investigate the tolerance against stress in Porphyra, we isolated the cyclophilin PhCYP18 gene (Accession number JQ413239) and measured its expression over different generations and stress conditions. In P. haitanensis, cyclophilin PhCYP18 accumulated more in the filamentous sporophyte generation than in the blade gametophyte generation. This difference was thought to be due to harsh environments and a gene dosage effect. It has been found, however, that PhCYP18 expression was dysregulated in blades under high salt stress, strong irradiance stress and multifactorial stress compared to blades under normal conditions. Moreover, the changes were not linearly related to the degree of stress. It was therefore thought that PhCYP18 actively responded to stress situations and induced strong stress tolerance, which is evident in P. haitanensis.
Bibliography:National Natural Science Foundation of China - No. 30830015; No. 30970302; No. 40806063; No. B49082401
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Fig. S1 Nucleotide and deduced amino acid sequence of PhCYP18 gene. Nucleotide residues are numbered in the 5′ to 3′ derection. Amino acid residues are numbered in the N- to C-terminal direction. Astericks denote the stop codon.Fig. S2 Multiple sequence alignment of cyclophilins in different species. Single domain cyclophilins were chose from different species and aligned by Clustal X (1.83) and Bioedit. Black shade means 100% conservation. Gray shade means 80% conservation. Asterisks means active site. Sequence in frame means basic cyclophilin ABH_like domain. The accession numbers of these sequences were showed as follow: Zea mays (CAA48638), Oryza sativa (AAA57046), Porphyra haitanensis (JQ413239), Chlamydomonas reinhardtii (AAC05639), Hemicentrotus pulcherrimus (P91791), Pinus massoniana (ACV88654), Griffithsia japonica (AAC64933).Fig. S3 Standard curve and melt peak chart of PhCYP18. (A) standard curve of samples G, S2, L2, STL1, STL2 and STL3 (B) standard curve of samples G, S1, S3, L1 and L3 (C) standard curve of samples C and G (D) melt peak chart of samples G, S2, L2, STL1, STL2 and STL3 (E) melt peak chart of samples G, S1, S3, L1 and L3 (F) melt peak chart of samples C and G.
ObjectType-Article-1
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content type line 23
ISSN:0022-3646
1529-8817
DOI:10.1111/j.1529-8817.2012.01234.x