Functional analysis of human NK cells by flow cytometry

Natural killer (NK) cells are a subset of lymphocytes that contribute to innate immunity through cytokine secretion and target cell lysis. NK cell function is regulated by a multiplicity of activating and inhibitory receptors. The advance in instrumentation for multi-color flow cytometry and the gen...

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Published inMethods in molecular biology (Clifton, N.J.) Vol. 612; p. 335
Main Authors Bryceson, Yenan T, Fauriat, Cyril, Nunes, João M, Wood, Stephanie M, Björkström, Niklas K, Long, Eric O, Ljunggren, Hans-Gustaf
Format Journal Article
LanguageEnglish
Published United States 2010
Subjects
Animals
Cell Degranulation
Cell Line
Cytokines - biosynthesis
Flow Cytometry - methods
Humans
Killer Cells, Natural - cytology
Killer Cells, Natural - immunology
Killer Cells, Natural - metabolism
Time Factors
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Summary:Natural killer (NK) cells are a subset of lymphocytes that contribute to innate immunity through cytokine secretion and target cell lysis. NK cell function is regulated by a multiplicity of activating and inhibitory receptors. The advance in instrumentation for multi-color flow cytometry and the generation of specific mAbs for different epitopes related to phenotypic and functional parameters have facilitated our understanding of NK cell responses. Here, we provide protocols for flow cytometric evaluation of degranulation and cytokine production by human NK cells from peripheral blood at the single-cell level. In addition to offering insight into the regulation of human NK cell responses, these techniques are applicable to the assessment of various clinical conditions, including the diagnosis of immunodeficiency syndromes.
ISSN:1940-6029
DOI:10.1007/978-1-60761-362-6_23