Binding of HBs Antigen to HeLa Cells by Use of Reconstitution into Liposomes and Fusion by Sendai Virus

Hepatitis B surface antigen (HBsAg) was reconstituted into liposomes composed of phosphatidylserin. An isolated membrane fraction of HeLa cells was mixed with the liposomes, then the liposomes were incubated with HeLa cells in the presence of Sendai virus. In this system the attachment of HBsAg to t...

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Bibliographic Details
Published inMicrobiology and immunology Vol. 26; no. 6; pp. 487 - 496
Main Authors Tanabe, Kiyokatsu, Naruto, Hiroshi, Shimizu, Yoshitaka
Format Journal Article
LanguageEnglish
Published Australia Blackwell Publishing Ltd 01.01.1982
Subjects
Antigens, Surface
Cell surface
HeLa Cells - immunology
HeLa Cells - metabolism
HeLa Cells - ultrastructure
Hepatitis B Surface Antigens - immunology
Hepatitis B virus
Humans
Liposomes - immunology
Microscopy, Electron
Parainfluenza Virus 1, Human - immunology
Sendai virus
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Summary:Hepatitis B surface antigen (HBsAg) was reconstituted into liposomes composed of phosphatidylserin. An isolated membrane fraction of HeLa cells was mixed with the liposomes, then the liposomes were incubated with HeLa cells in the presence of Sendai virus. In this system the attachment of HBsAg to the cell surface was enhanced and became resistant to treatment with trypsin‐EDTA. The presence of the HBsAg on the cell surface was revealed by immunoelectron‐microscopy. This technique may provide a model system for studying immunological reactions to HBsAg‐bearing target cells in vitro.
Bibliography:istex:4B18F5D1E48A12FD82815CF4723C11B05F35FE16
ArticleID:MIM00202
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ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1982.tb00202.x