Stabilizing Antibody Cocktails for Mass Cytometry

Mass cytometry is increasingly employed in larger immune profiling studies involving data acquisitions across several days and multiple sites. For gaining a maximum of information from respective data by computational analyses, several techniques have been developed to minimize noise in mass cytomet...

Full description

Saved in:
Bibliographic Details
Published inCytometry. Part A Vol. 95; no. 8; pp. 910 - 916
Main Authors Schulz, Axel R., Baumgart, Sabine, Schulze, Julia, Urbicht, Marie, Grützkau, Andreas, Mei, Henrik E.
Format Journal Article
LanguageEnglish
Published Hoboken, USA John Wiley & Sons, Inc 01.08.2019
Wiley Subscription Services, Inc
Subjects
Antibodies
antibody cocktail
Computer applications
Cryopreservation
CyTOF
Cytometry
Data acquisition
data consistency
immune monitoring
immune phenotyping
mass cytometry
multiplexing
Palladium
Platinum
Staining
multiplexing
antibody cocktail
immune monitoring
mass cytometry
CyTOF
cryopreservation
data consistency
immune phenotyping
Online AccessGet full text

Cover

More Information
Summary:Mass cytometry is increasingly employed in larger immune profiling studies involving data acquisitions across several days and multiple sites. For gaining a maximum of information from respective data by computational analyses, several techniques have been developed to minimize noise in mass cytometric data sets, such as sample banking, standardized instrument setup, sample barcoding, and signal normalization. However, the repeated preparation of cocktails composed of isotope‐tagged antibodies remained a significant source of error. We here show that premixed antibody cocktails fail to deliver expected staining patterns when stored at 4°C for 4 weeks. As a solution, we developed and tested a cryopreservation method for highly multiplexed antibody cocktails for mass cytometry including lanthanide, palladium, and platinum conjugates that yielded stable staining patterns for at least 9 months when stored at temperatures below −80°C. Using frozen aliquots of antibody cocktails is an economic and flexible approach to significantly improve data consistency in large mass cytometry studies with repetitive staining/measurement cycles spanning several days or involving multiple data acquisition sites. © 2019 International Society for Advancement of Cytometry
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:1552-4922
1552-4930
1552-4930
DOI:10.1002/cyto.a.23781