Glycan Stimulation Enables Purification of Prostate Cancer Circulating Tumor Cells on PEDOT NanoVelcro Chips for RNA Biomarker Detection

• Published in: Advanced healthcare materials Vol. 7; no. 3
• Main Authors: Shen, Mo-Yuan, Chen, Jie-Fu, Luo, Chun-Hao, Lee, Sangjun, Li, Cheng-Hsuan, Yang, Yung-Ling, Tsai, Yu-Han, Ho, Bo-Cheng, Bao, Li-Rong, Lee, Tien-Jung, Jan, Yu Jen, Zhu, Ya-Zhen, Cheng, Shirley, Feng, Felix Y, Chen, Peilin, Hou, Shuang, Agopian, Vatche, Hsiao, Yu-Sheng, Tseng, Hsian-Rong, Posadas, Edwin M, Yu, Hsiao-Hua
• Format: Journal Article
• Language: English
• Published: Germany 01.02.2018
• Subjects: Biomarkers - analysis; Bridged Bicyclo Compounds, Heterocyclic - chemistry; Cell Line, Tumor; Humans; Male; Nanostructures - chemistry; Neoplastic Cells, Circulating - metabolism; Neoplastic Cells, Circulating - pathology; Polymers - chemistry; Prostatic Neoplasms - metabolism; Prostatic Neoplasms - pathology; RNA - analysis; prostate cancer; RNA biomarkers; circulating tumor cells; poly(3,4-ethylene-dioxythiophene)s; responsive materials
• ISSN: 2192-2659
• DOI: 10.1002/adhm.201700701

A glycan-stimulated and poly(3,4-ethylene-dioxythiophene)s (PEDOT)-based nanomaterial platform is fabricated to purify circulating tumor cells (CTCs) from blood samples of prostate cancer (PCa) patients. This new platform, phenylboronic acid (PBA)-grafted PEDOT NanoVelcro, combines the 3D PEDOT nanosubstrate, which greatly enhances CTC capturing efficiency, with a poly(EDOT-PBA-co-EDOT-EG3) interfacial layer, which not only provides high specificity for CTC capture upon antibody conjugation but also enables competitive binding of sorbitol to gently release the captured cells. CTCs purified by this PEDOT NanoVelcro chip provide well-preserved RNA transcripts for the analysis of the expression level of several PCa-specific RNA biomarkers, which may provide clinical insights into the disease.