Genotoxicity testing of cigarette-smoke condensate in the SCE and HGPRT assays with V79 Chinese hamster cells

• Published in: Food and chemical toxicology Vol. 23; no. 6; pp. 603 - 607
• Main Authors: Jongen, W.M.F., Hakkert, B.C., van der Hoeven, J.C.M.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.01.1985; New York, NY Elsevier Science
• Subjects: Animals; Biological and medical sciences; Biotransformation; Cells, Cultured; Chemical mutagenesis; Chick Embryo; Cricetinae; Cricetulus; Dose-Response Relationship, Drug; Hypoxanthine Phosphoribosyltransferase - genetics; Leerstoelgroep Toxicologie; Male; Medical sciences; Mutagenicity Tests; Mutagens - metabolism; Nicotiana; Plants, Toxic; Rats; Rats, Inbred Strains; Sister Chromatid Exchange - drug effects; Smoke - adverse effects; Sub-department of Toxicology; Toxicologie; Toxicology; CSC = cigarette-smoke condensate; HGPRT = hypoxanthine-guanine phosphoribosyl transferase; TPA = tetradecanoic acid phorbolester; B[ a]P = benzo[ a]pyrene; DMSO = dimethylsulphoxide; SCE = sister-chromatid exchange; 6TG = 6-thioguanine; Vertebrata; Tobacco; Mammalia; Cell line; Mutagenicity testing; Sister chromatid exchange; Rodentia; Hypoxanthine phosphoribosyltransferase; Fibroblast; Fumes; Hamster
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/0278-6915(85)90186-3

The genotoxicity of cigarette-smoke condensate (CSC) was investigated using V79 Chinese hamster fibroblasts in the sister-chromatid exchange (SCE) and HGPRT (hypoxanthine-guanine-phosphoribosyl-transferase) tests. CSC was negative in the SCE test both with and without metabolic activation (co-cultivation with chick-embryo primary hepatocytes). In the HGPRT test no direct mutagenicity of CSC was observed but after metabolic activation there was a considerable increase in the number of mutants. Comparison of different metabolizing systems showed that non-induced chick hepatocytes, liver homogenate from non-induced chick embryos and liver homogenate from rats pretreated with Aroclor 1254 generated similar numbers of mutants in cells treated with CSC. In addition the capacity of CSC to inhibit metabolic co-operation between V79 cells was studied. A dose-related increase in the inhibition of metabolic co-operation was observed.