Pancreatic enzyme deficiency depends on dietary protein origin in milk-fed calves

• Published in: Journal of dairy science Vol. 94; no. 3; pp. 1517 - 1525
• Main Authors: Guilloteau, P., Plodari, M., Romé, V., Savary, G., Le Normand, L., Zabielski, R.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.03.2011; Elsevier; American Dairy Science Association
• Subjects: Agricultural sciences; Animal Feed; Animal Nutritional Physiological Phenomena; Animal productions; Animals; Biological and medical sciences; calves; cannulas; Cattle - metabolism; Cattle - physiology; dairy protein; dietary protein origin; digestibility; digestion; Digestion - physiology; dried skim milk; Feces - chemistry; Food industries; Fundamental and applied biological sciences. Psychology; humans; Life Sciences; Male; milk; Milk - chemistry; Milk and cheese industries. Ice creams; milk proteins; Milk Proteins - administration & dosage; Milk Proteins - metabolism; Pancreas - enzymology; pancreatic juice; Pancreatic Juice - enzymology; pancreatic juice secretion; Soybean Proteins - administration & dosage; Soybean Proteins - metabolism; soybeans; Terrestrial animal productions; trypsin; trypsin activity; Vertebrates; pancreatic juice secretion; trypsin activity; digestibility; dietary protein origin; Origin; Serine endopeptidases; Animal feeding; Enzyme; Secretion; Deficiency; Dairy product; Pancreatic juice; Protein; Peptidases; Trypsin; Dairy industry; Hydrolases; Digestibility; Milk; PANCREATIC JUICE SECRETION; DIETARY PROTEIN ORIGIN; TRYPSIN ACTIVITY; DIGESTIBILITY
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.2010-3906

In young mammals, milk proteins and their substitutes are used in milk formula. Protein substitution modifies diet digestibility and pancreatic secretions. The aim of this study was to test if milk protein substitution could generate pancreatic deficiency in milk-fed calves. The effect of pancreatic juice on the digestibility of proteins was studied. Measurement of apparent fecal nutrient digestibility was used to estimate digestion. Ten calves (60 to 130 d old) were chronically fitted with pancreatic accessory duct cannulas and 2 duodenal cannulas to provide precise measurement, sampling, and reintroduction of pancreatic juice as well as additional infusions. Animals were fed milk formula based on skim milk powder or soybean concentrate. Level of deficiency depended on dietary protein origin. Twice as much protein or trypsin was required with a soybean concentrate diet than with a skim milk powder diet to obtain maximal nutrient digestibility. Pancreatic protein concentration in the juice can be used to differentiate between normal and deficient animals. Among these proteins, trypsin measurement is a good pancreatic deficiency marker. These results confirmed the major role of exocrine pancreatic secretions in producing optimal digestion in young calves. Furthermore, practical applications of these results can be applied for the young in other animal species and in humans.