ScMT2-1-3, a Metallothionein Gene of Sugarcane, Plays an Important Role in the Regulation of Heavy Metal Tolerance/Accumulation

• Published in: BioMed research international Vol. 2013; no. 2013; pp. 1 - 12
• Main Authors: You-Xiong, Que, Gao, Shiwu, Wang, Hengbo, Su, Yachun, Li-Ping, Xu, Guo, Jinlong, Xu, Jingsheng
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2013; Hindawi Limited
• Subjects: Adaptation, Physiological - drug effects; Adaptation, Physiological - genetics; Amino Acid Sequence; Base Sequence; Bioinformatics; Biology; Biomedical research; Cadmium; Cloning; Cloning, Molecular; E coli; Escherichia coli - growth & development; Escherichia coli - metabolism; Gene Expression Profiling; Gene Expression Regulation, Plant - drug effects; Genes; Genes, Plant - genetics; Metallothionein - genetics; Metals, Heavy - toxicity; Molecular Sequence Data; Molecular weight; Physical properties; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Recombinant Proteins - isolation & purification; Saccharum - drug effects; Saccharum - genetics; Saccharum - physiology; Sequence Alignment; Sequence Analysis, DNA; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Stress, Physiological - drug effects; Stress, Physiological - genetics; Toxicity; China
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2013/904769

Plant metallothioneins (MTs), which are cysteine-rich, low-molecular-weight, and metal-binding proteins, play important roles in detoxification, metal ion homeostasis, and metal transport adjustment. In this study, a novel metallothionein gene, designated as ScMT2-1-3 (GenBank Accession number JQ627644), was identified from sugarcane. ScMT2-1-3 was 700 bp long, including a 240 bp open reading frame (ORF) encoding 79 amino acid residues. A His-tagged ScMT2-1-3 protein was successfully expressed in Escherichia coli system which had increased the host cell’s tolerance to Cd2+, Cu2+, PEG, and NaCl. The expression of ScMT2-1-3 was upregulated under Cu2+ stress but downregulated under Cd2+ stress. Real-time qPCR demonstrated that the expression levels of ScMT2-1-3 in bud and root were over 14 times higher than those in stem and leaf, respectively. Thus, both the E. coli assay and sugarcane plantlets assay suggested that ScMT2-1-3 is significantly involved in the copper detoxification and storage in the cell, but its functional mechanism in cadmium detoxification and storage in sugarcane cells needs more testification though its expressed protein could obviously increase the host E. coli cell’s tolerance to Cd2+. ScMT2-1-3 constitutes thus a new interesting candidate for elucidating the molecular mechanisms of MTs-implied plant heavy metal tolerance/accumulation and for developing sugarcane phytoremediator varieties.