Transpulmonary cardiac output measurement in a rat model of cardiac arrest and CPR: Impact of vascular access

• Published in: Resuscitation Vol. 81; no. 2; pp. 248 - 254
• Main Authors: Kelm, Robert F, Wagenführer, Jürgen, Schmidtmann, Irene, Engelhard, Kristin, Werner, Christian, Noppens, Rüdiger R
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.02.2010; Elsevier
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Asphyxia; Biological and medical sciences; Brain injury; Cardiac arrest; Cardiac Output; Cardiopulmonary Resuscitation; Cerebral blood flow; Disease Models, Animal; Emergency; Emergency and intensive cardiocirculatory care. Cardiogenic shock. Coronary intensive care; Heart Arrest - physiopathology; Heart Arrest - therapy; Heart failure; Heart Function Tests - methods; Intensive care medicine; Male; Medical sciences; Rats; Rats, Sprague-Dawley; Heart failure; Cerebral blood flow; Cardiopulmonary resuscitation; Cardiac arrest; Asphyxia; Cardiac output; Brain injury; Intensive care; Rat; Central nervous system; Cardiovascular disease; Encephalon; Heart disease; Intensive cardiocirculatory care; Nervous system diseases; Cardiocirculatory arrest; Respiratory disease; Rodentia; Blood flow; Cerebral disorder; Vertebrata; Mammalia; Animal; Central nervous system disease; Hemodynamics
• ISSN: 0300-9572; 1873-1570
• DOI: 10.1016/j.resuscitation.2009.10.024

Abstract Objective The present study investigated the impact of the vascular access site for cardiac output (CO) measurement by thermodilution on survival and neurohistopathological injury in a rat model of cardiac arrest (CA) and cardiopulmonary resuscitation (CPR). Secondary the influence of the vascular access site on cardiac output measurements was examined. Methods Rats underwent asphyxial CA and CPR. Thermocouple probes were either placed via the femoral artery into the bifurcation of abdominal aorta/iliac artery (Femoral) or via the carotid artery into the aortic arch (Carotid). CPR was initiated after 9 min CA. Local cerebral blood flow (lCBF) and CO were assessed for 120 min after restoration of spontaneous circulation. Neurohistopathological injury was determined using Fluoro-Jade B staining. Results Survival was reduced in the Carotid group compared to the Femoral group ( p < 0.01). Fluoro-Jade B staining in the hippocampus showed no difference between CA groups. CO measurements were comparable between femoral and carotid artery access sites. lCBF revealed a delayed hyperperfusion in the Carotid group only. Conclusions The present study demonstrates the influence of the vascular access site for placing thermocouple probes for CO measurement on animal survival after CA/CPR. CO did not differ between the two access sites with consequential different detection sites. Use of the femoral access for CO measurement is recommended for long-term survival after CA/CPR.