Rapid Bedside Inactivation of Ebola Virus for Safe Nucleic Acid Tests

Rapid bedside inactivation of Ebola virus would be a solution for the safety of medical and technical staff, risk containment, sample transport, and high-throughput or rapid diagnostic testing during an outbreak. We show that the commercially available Magna Pure lysis/binding buffer used for nuclei...

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Published inJournal of clinical microbiology Vol. 54; no. 10; pp. 2521 - 2529
Main Authors Rosenstierne, Maiken Worsøe, Karlberg, Helen, Bragstad, Karoline, Lindegren, Gunnel, Stoltz, Malin Lundahl, Salata, Cristiano, Kran, Anne-Marte Bakken, Dudman, Susanne Gjeruldsen, Mirazimi, Ali, Fomsgaard, Anders
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.10.2016
Subjects
Disinfection - methods
Ebolavirus
Hemorrhagic Fever, Ebola - diagnosis
Hemorrhagic Fever, Ebola - virology
Humans
Medicin och hälsovetenskap
Point-of-Care Systems
RNA, Viral - isolation & purification
Specimen Handling - methods
Temperature
Time Factors
Virology
Virus Inactivation
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Summary:Rapid bedside inactivation of Ebola virus would be a solution for the safety of medical and technical staff, risk containment, sample transport, and high-throughput or rapid diagnostic testing during an outbreak. We show that the commercially available Magna Pure lysis/binding buffer used for nucleic acid extraction inactivates Ebola virus. A rapid bedside inactivation method for nucleic acid tests is obtained by simply adding Magna Pure lysis/binding buffer directly into vacuum blood collection EDTA tubes using a thin needle and syringe prior to sampling. The ready-to-use inactivation vacuum tubes are stable for more than 4 months, and Ebola virus RNA is preserved in the Magna Pure lysis/binding buffer for at least 5 weeks independent of the storage temperature. We also show that Ebola virus RNA can be manually extracted from Magna Pure lysis/binding buffer-inactivated samples using the QIAamp viral RNA minikit. We present an easy and convenient method for bedside inactivation using available blood collection vacuum tubes and reagents. We propose to use this simple method for fast, safe, and easy bedside inactivation of Ebola virus for safe transport and routine nucleic acid detection.
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Citation Rosenstierne MW, Karlberg H, Bragstad K, Lindegren G, Stoltz ML, Salata C, Kran A-MB, Dudman SG, Mirazimi A, Fomsgaard A. 2016. Rapid bedside inactivation of Ebola virus for safe nucleic acid tests. J Clin Microbiol 54:2521–2529. doi:10.1128/JCM.00346-16.
A.M. and A.F. contributed equally to this article.
ISSN:0095-1137
1098-660X
1098-660X
DOI:10.1128/JCM.00346-16