2,3,7,8-Tetrachlorodibenzo-p-dioxin exposure disrupts development of the visceral and ocular vasculature

• Published in: Aquatic toxicology Vol. 234; p. 105786
• Main Authors: Yue, Monica S., Martin, Shannon E., Martin, Nathan R., Taylor, Michael R., Plavicki, Jessica S.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2021
• Subjects: 2,3,7,8-Tetrachlorodibenzo-p-dioxin; Animals; Animals, Genetically Modified - metabolism; Bone Morphogenetic Protein 4 - genetics; Bone Morphogenetic Protein 4 - metabolism; Development; Dioxin; Embryo, Nonmammalian - drug effects; Embryo, Nonmammalian - metabolism; Gut; Kidney; Liver; Liver - blood supply; Pancreas; Polychlorinated Dibenzodioxins - toxicity; Retina; Retinal Vein - drug effects; Retinal Vein - growth & development; SAV; SIVP; Subintestinal venous plexus; Superficial annular vessel; TCDD; Vascular development; Vasculature; Veins - drug effects; Water Pollutants, Chemical - toxicity; Zebrafish; Zebrafish - growth & development; Zebrafish - metabolism; Zebrafish Proteins - genetics; Zebrafish Proteins - metabolism; Superficial annular vessel; Liver; Gut; TCDD; SAV; Zebrafish; Retina; 2,3,7,8-Tetrachlorodibenzo-p-dioxin; Vascular development; Kidney; Vasculature; Subintestinal venous plexus; Dioxin; SIVP; Development; Pancreas
• ISSN: 0166-445X; 1879-1514
• DOI: 10.1016/j.aquatox.2021.105786

•Dioxin disrupts the development of the subintestinal venous plexus (SIVP).•The SIVP vascularizes the developing liver, kidney, gut, and pancreas.•Dioxin disrupts development of the superficial annular vessel (SAV) in the retina.•The observed vascular phenotypes are not secondary to changes in circulation.•Dioxin increases the expression of bmp4, a key mediator of SIVP morphogenesis. The aryl hydrocarbon receptor (AHR) has endogenous functions in mammalian vascular development and is necessary for mediating the toxic effects of a number of environmental contaminants. Studies in mice have demonstrated that AHR is necessary for the formation of the renal, retinal, and hepatic vasculature. In fish, exposure to the prototypic AHR agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induces expression of the AHR biomarker cyp1a throughout the developing vasculature and produces vascular malformations in the head and heart. However, it is not known whether the vascular structures that are sensitive to loss of AHR function are also disrupted by aberrant AHR activation. Here, we report that TCDD-exposure in zebrafish disrupts development of 1) the subintestinal venous plexus (SIVP), which vascularizes the developing liver, kidney, gut, and pancreas, and 2) the superficial annular vessel (SAV), an essential component of the retinal vasculature. Furthermore, we determined that TCDD exposure increased the expression of bmp4, a key molecular mediator of SIVP morphogenesis. We hypothesize that the observed SIVP phenotypes contribute to one of the hallmarks of TCDD exposure in fish – the failure of the yolk sac to absorb. Together, our data describe novel TCDD-induced vascular phenotypes and provide molecular insight into critical factors producing the observed vascular malformations.