Effects of lysophosphatidylcholine on β-amyloid-induced neuronal apoptosis
Aim: We have investigated the effects of lysophosphatidylcholine (LPC), a product of lipid peroxidation, on Aβ1-42-induced SH-SY5Y cell apoptosis. Methods: The viability of cultured SH-SY5Y cells was measured using a CCK-8 kit. Apoptosis was determined by Chipased flow cytometric assay. The mRNA tra...
Saved in:
Published in | Acta pharmacologica Sinica Vol. 30; no. 4; pp. 388 - 395 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group UK
01.04.2009
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Aim: We have investigated the effects of lysophosphatidylcholine (LPC), a product of lipid peroxidation, on Aβ1-42-induced SH-SY5Y cell apoptosis. Methods: The viability of cultured SH-SY5Y cells was measured using a CCK-8 kit. Apoptosis was determined by Chipased flow cytometric assay. The mRNA transcription of Bcl-2, Bax, and caspase-3 were detected by using reverse transcription and real-time quantitative PCR and the protein levels of Bax and caspase-3 were analyzed by Western blotting. The cytosolic calcium concentration of SH-SY5Y cells was tested by calcium influx assay. G2A expression in SH-SY5Y cells was silenced by small interfering RNA.
Results: Long-term exposure of SH-SY5Y cells to LPC augmented the neurotoxicity of Aβ1-42, Furthermore, after LPC treatment, the Bax/Bcl-XL ratio and the expression levels, as well as the activity of caspase-3 were, elevated, whereas the expression level of TRAF1 was reduced. Because LPC was reported to be a specific ligand for the orphan G-protein coupled receptor, G2A, we investigated LPC-mediated changes in calcium levels in SH-SY5Y cells. Our results demonstrated that LPC can enhance the Aβ1-42-induced elevation of intracellular calcium. Interestingly, Aβ1-42 significantly increased the expression of G2A in SH-SY5Y cells, whereas knockdown of G2A using siRNA reduced the effects of LPC on Aβ1-42induced neurotoxicity. Conclusion: The effects of LPC on Aβ1-42-induced apoptosis may occur through the signal pathways of the orphan G-protein coupled receptor. |
---|---|
Bibliography: | lysophosphatidylcholine amyloid beta (1-42) peptide R743.31 amyloid beta (1-42) peptide; lysophosphatidylcholine; neuronal apoptosis R285.5 neuronal apoptosis 31-1347/R |
ISSN: | 1671-4083 1745-7254 |
DOI: | 10.1038/aps.2009.25 |