MAU-8 is a Phosducin-like Protein required for G protein signaling in C. elegans
The mau-8(qm57) mutation inhibits the function of GPB-2, a heterotrimeric G protein β subunit, and profoundly affects behavior through the Gαq/Gαo signaling network in C. elegans. mau-8 encodes a nematode Phosducin-like Protein (PhLP), and the qm57 mutation leads to the loss of a predicted phosphory...
Saved in:
Published in | Developmental biology Vol. 294; no. 1; pp. 181 - 191 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
01.06.2006
Elsevier |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The
mau-8(qm57) mutation inhibits the function of GPB-2, a heterotrimeric G protein β subunit, and profoundly affects behavior through the Gαq/Gαo signaling network in
C. elegans.
mau-8 encodes a nematode Phosducin-like Protein (PhLP), and the
qm57 mutation leads to the loss of a predicted phosphorylation site in the C-terminal domain of PhLP that binds the Gβγ surface implicated in membrane interactions. In developing embryos, MAU-8/PhLP localizes to the cortical region, concentrates at the centrosomes of mitotic cells and remains associated with the germline blastomere. In adult animals, MAU-8/PhLP is ubiquitously expressed in somatic tissues and germline cells. MAU-8/PhLP interacts with the PAR-5/14.3.3 protein and with the Gβ subunit GPB-1. In
mau-8 mutants, the disruption of MAU-8/PhLP stabilizes the association of GPB-1 with the microtubules of centrosomes. Our results indicate that MAU-8/PhLP modulates G protein signaling, stability and subcellular location to regulate various physiological functions, and they suggest that MAU-8 might not be limited to the Gαq/Gαo network. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0012-1606 1095-564X |
DOI: | 10.1016/j.ydbio.2006.02.039 |