Chaperonin-Containing t-Complex Protein-1 Subunit β as a Possible Biomarker for the Phase of Glomerular Hyperfiltration of Diabetic Nephropathy

• Published in: Disease markers Vol. 2015; no. 2015; pp. 1 - 7
• Main Authors: Pei, Dee, Hung, Yi-Jen, Lin, Yuh-Feng, Chang, Li-Chien, Wu, Chung-Ze, Chen, Jin-Shuen
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2015; John Wiley & Sons, Inc
• Subjects: Abnormalities; Aged; Animals; Biomarkers - metabolism; Biomarkers - urine; Case-Control Studies; Chaperonin Containing TCP-1 - genetics; Chaperonin Containing TCP-1 - metabolism; Chaperonin Containing TCP-1 - urine; Development and progression; Diabetic nephropathies; Diabetic Nephropathies - metabolism; Diabetic Nephropathies - urine; Female; Genetic aspects; Glomerular Filtration Rate; Health aspects; Humans; Kidney - metabolism; Kidney - physiopathology; Male; Mice; Mice, Inbred BALB C; Middle Aged; Molecular chaperones
• ISSN: 0278-0240; 1875-8630
• DOI: 10.1155/2015/548101

In cell model, we discovered the association between chaperonin-containing t-complex polypeptide 1 subunit β (TCP-1β) and early diabetic nephropathy (DN). In this study, we further explored the relationships between TCP-1β and type 2 diabetic mellitus (DM). To mimic the clinical hyperfiltration state, a type 2 DM mice model was established by feeding a high-fat diet in combination with treatment of streptozotocin and nicotinamide. Blood and urine were collected to determine creatinine clearance (Ccr), and kidney tissues were harvested for evaluation of TCP-1β expression by immunohistochemistry and Western blot. Meanwhile, clinical subjects of healthy controls and type 2 DM were recruited to strengthen the evidence with urine TCP-1β. Results showed that Ccr and the expression of TCP-1β in kidney were significantly higher one week after hyperglycemia development, suggesting that the hyperfiltration state was successfully established in the mice model. TCP-1β was expressed predominantly on renal tubules. By using the estimated glomerular filtration rate to index progression in clinical investigation, urine TCP-1β level was associated with the hyperfiltration phase in type 2 DM patients. Conclusively, we confirmed that TCP-1β is a possible biomarker for early nephropathy of type 2 DM, but further mechanistic study to elucidate its cause and pathway is needed.