The Calmodulin Binding Region of the Synaptic Vesicle Protein Mover Is Required for Homomeric Interaction and Presynaptic Targeting
The synaptic vesicle associated protein Mover / TPRGL / SVAP30 is one of the few vertebrate-specific proteins in the evolutionarily conserved machinery mediating neurotransmitter release. Little is known about its molecular properties and how it may interact with the conserved components of the pres...
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Published in | Frontiers in molecular neuroscience Vol. 12; p. 249 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Lausanne
Frontiers Research Foundation
08.11.2019
Frontiers Media S.A |
Subjects | |
Online Access | Get full text |
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Summary: | The synaptic vesicle associated protein Mover / TPRGL / SVAP30 is one of the few vertebrate-specific proteins in the evolutionarily conserved machinery mediating neurotransmitter release. Little is known about its molecular properties and how it may interact with the conserved components of the presynaptic machinery. Here, we show by deletion analysis that regions required for homomeric interaction of Mover are distributed across the entire molecule, including N-terminal, central and C-terminal regions. The same regions are also required for the accumulation of Mover in presynaptic terminals of cultured neurons. Mutating two phosphorylation sites in N-terminal regions did not affect these properties. In contrast, a point mutation in the predicted Calmodulin binding sequence of Mover abolished both homomeric interaction and presynaptic targeting. We show that this sequence indeed binds Calmodulin, and that recombinant Mover increases Calmodulin-signaling upon heterologous expression. Our data suggest that presynaptic accumulation of Mover requires homomeric interaction mediated by regions distributed across large areas of the protein, and corroborate the hypothesis that Mover functionally interacts with Calmodulin-signaling. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Vsevolod V. Gurevich, Vanderbilt University, United States Reviewed by: Silvia Giovedi, University of Genoa, Italy; Shasta Sabo, Central Michigan University, United States |
ISSN: | 1662-5099 1662-5099 |
DOI: | 10.3389/fnmol.2019.00249 |