Identification of New Intrinsic Proteins in Arabidopsis Plasma Membrane Proteome
Identification and characterization of anion channel genes in plants represent a goal for a better understanding of their central role in cell signaling, osmoregulation, nutrition, and metabolism. Though channel activities have been well characterized in plasma membrane by electrophysiology, the cor...
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Published in | Molecular & cellular proteomics Vol. 3; no. 7; pp. 675 - 691 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Society for Biochemistry and Molecular Biology
01.07.2004
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Subjects | |
Online Access | Get full text |
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Summary: | Identification and characterization of anion channel genes in plants represent a goal for a better understanding of their
central role in cell signaling, osmoregulation, nutrition, and metabolism. Though channel activities have been well characterized
in plasma membrane by electrophysiology, the corresponding molecular entities are little documented. Indeed, the hydrophobic
protein equipment of plant plasma membrane still remains largely unknown, though several proteomic approaches have been reported.
To identify new putative transport systems, we developed a new proteomic strategy based on mass spectrometry analyses of a
plasma membrane fraction enriched in hydrophobic proteins. We produced from Arabidopsis cell suspensions a highly purified plasma membrane fraction and characterized it in detail by immunological and enzymatic
tests. Using complementary methods for the extraction of hydrophobic proteins and mass spectrometry analyses on mono-dimensional
gels, about 100 proteins have been identified, 95% of which had never been found in previous proteomic studies. The inventory
of the plasma membrane proteome generated by this approach contains numerous plasma membrane integral proteins, one-third
displaying at least four transmembrane segments. The plasma membrane localization was confirmed for several proteins, therefore
validating such proteomic strategy. An in silico analysis shows a correlation between the putative functions of the identified proteins and the expected roles for plasma
membrane in transport, signaling, cellular traffic, and metabolism. This analysis also reveals 10 proteins that display structural
properties compatible with transport functions and will constitute interesting targets for further functional studies. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1535-9476 1535-9484 |
DOI: | 10.1074/mcp.M400001-MCP200 |