A versatile ultra-high performance LC-MS method for lipid profiling

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 951-952; pp. 119 - 128
• Main Authors: Knittelfelder, Oskar L., Weberhofer, Bernd P., Eichmann, Thomas O., Kohlwein, Sepp D., Rechberger, Gerald N.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2014; Elsevier
• Subjects: Animals; Chromatography, High Pressure Liquid - methods; Glycerophospholipids; Lipidomics; Lipids - analysis; Lipids - chemistry; Mass spectrometry; Mass Spectrometry - methods; Mice; Muscles - chemistry; Neutral lipids; Phosphoric Acids; Ultra-performance liquid chromatography; Yeasts - chemistry; C/M; DG; PS; Glycerophospholipids; SE; Ultra-performance liquid chromatography; Neutral lipids; ESI; SM; WT; MM; BMP; Lipidomics; CL; GP; TIC; Cer; PA; PC; TG; XIC; PE; PG; PI; Mass spectrometry; NL
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2014.01.011

•UPLC-MSE based method for lipid separation and identification.•The method is suitable for polar and non-polar lipid species.•Excellent separation of lipid species within lipid classes.•Identification of low abundant lipid species.•May be combined with ESI-MS both in positive and negative ionization mode. A new UPLC-based untargeted lipidomic approach using a qTOF hybrid mass spectrometer is introduced. The applied binary gradient enables separations of lipid species including constitutional isomeric compounds and low abundant lipid classes such as phosphatidic acid (PA). Addition of phosphoric acid to the solvents improves peak shapes for acidic phospholipids. MSE scans allow simultaneous acquisition of full scan data and collision induced fragmentation to improve identification of lipid classes and to obtain structural information. The method was used to investigate the lipidome of yeast.