Allergy to the heat-labile proteins α-lactalbumin and β-lactoglobulin in mare’s milk

• Published in: Journal of allergy and clinical immunology Vol. 97; no. 6; pp. 1304 - 1307
• Main Authors: Gall, Helmut, Kalveram, Christa Maria, Sick, Hinrich, Sterry, Wolfram
• Format: Journal Article
• Language: English
• Published: New York, NY Mosby, Inc 01.06.1996; Elsevier
• Subjects: Allergens - chemistry; Allergic diseases; Animals; Biological and medical sciences; Digestive allergic diseases; Female; Heat-labile allergy proteins; Horses; Hot Temperature; Humans; Immunopathology; Lactalbumin - immunology; Lactoglobulins - immunology; mare’s milk; Medical sciences; Middle Aged; Milk Hypersensitivity - immunology; Milk Proteins - immunology; Molecular Weight; Radioallergosorbent Test; Skin Tests; whey; α-lactalbumin; β-lactoglobulin; mare’s milk; SDS-PAGE; Heat-labile allergy proteins; whey; TBS; α-lactalbumin; β-lactoglobulin; MW; Human; Immunopathology; Allergy; Radioallergosorbent test; Etiopathogenesis; Mare milk; Case study; Lactoglobulins; Lactalbumin; Immunoblotting assay; Digestive diseases; Female; Skin test
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/S0091-6749(96)70199-9

BACKGROUND: Allergy to mare’s milk is rare. Recently, however, mare’s milk has been recommended for treatment of various ailments by practitioners of “alternative medicine,” and it is available in health food stores. OBJECTIVE: We report a case of allergic reaction to mare’s milk in a 51-year-old woman who was able to tolerate cow’s milk. METHODS: The protein composition of mare’s milk was determined by methods based on measurement of nitrogen content. The patient underwent prick and intracutaneous tests with commercially available bovine milk proteins and several mare’s milk preparations, including mare’s milk granulate and boiled mare’s milk. RAST and immunoblotting were also performed. RESULTS: Results of skin testing and RAST with cow’s milk were negative but demonstrated an IgE-mediated allergy to mare’s milk. Immunoblotting revealed two allergen bands with molecular weights of 16 and 18 kd, most likely representing the whey proteins α-lactalbumin and β-lactoglobulin. The bands disappeared after the mare’s milk was boiled, indicating that the proteins are heat-labile. CONCLUSION: The results of this study demonstrate the existence of an IgE-mediated mare’s milk allergy caused by low molecular weight heat-labile proteins, most likely α-lactalbumin and β-lactoglobulin, which do not cross-react with the corresponding whey proteins in cow’s milk. (J ALLERGY CLIN IMMUNOL 1996;97:1304-7.)