Developmental competence of frozen-thawed yak (Bos grunniens) oocytes followed by in vitro maturation and fertilization

In the present study, we examined the ability of immature germinal vesicle (GV) and subjected to in vitro matured (MII) yak oocytes to survive after cryopreservation as well as their subsequent development following in vitro maturation and fertilization. Both GV and MII oocytes were cryopreserved by...

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Published inCryobiology Vol. 68; no. 1; pp. 152 - 154
Main Authors Niu, Hui-Ran, Zi, Xiang-Dong, Xiao, Xiao, Xiong, Xian-Rong, Zhong, Jin-Cheng, Li, Jian, Wang, Li, Wang, Yong
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Inc 01.02.2014
Subjects
Animals
Blastocyst - cytology
Blastocyst - drug effects
Blastocyst - physiology
Bos grunniens
Cattle
Cell Survival - drug effects
Cryopreservation
Cryopreservation - methods
Cryoprotective Agents - pharmacology
Culture Media
Dimethyl Sulfoxide - pharmacology
Embryo, Mammalian
Embryonic Development - drug effects
Ethylene Glycol - pharmacology
Female
Fertilization - drug effects
Fertilization - physiology
Fertilization in Vitro
Ficoll - pharmacology
In vitro development
In vitro fertilization
In vitro maturation
Male
Oocytes - cytology
Oocytes - drug effects
Oocytes - physiology
Osmolar Concentration
Sucrose - pharmacology
Vitrification
Yak oocytes
Yak oocytes
In vitro fertilization
In vitro development
Cryopreservation
In vitro maturation
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Summary:In the present study, we examined the ability of immature germinal vesicle (GV) and subjected to in vitro matured (MII) yak oocytes to survive after cryopreservation as well as their subsequent development following in vitro maturation and fertilization. Both GV and MII oocytes were cryopreserved by using two different vitrification solutions (VS); VS-I contained 10% ethylene glycol (EG) and 10% dimethylsulfoxide (DMSO) in TCM-199+20% (v/v) fetal calf serum (FCS) whereas VS-II contained 40% EG+18% Ficoll+0.5M sucrose in TCM-199+20% FCS. The percentage of oocytes found to be morphologically normal was greater (P<0.01) in VS-I group than in VS-II group. Rates of cleavage (30.6–42.2%) and blastocyst formation (2.9–8.9%) did not differ among groups, but were lower than in unfrozen control (55.7% and 25.4%, P<0.01). These results show that a combination of EG and DMSO or EG, Ficoll and sucrose can be used to cryopreserve yak oocytes in French straws.
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ISSN:0011-2240
1090-2392
DOI:10.1016/j.cryobiol.2014.01.012