Human urine proteomics: building a list of human urine cancer biomarkers

• Published in: Expert review of proteomics Vol. 8; no. 3; pp. 347 - 360
• Main Authors: Casado-Vela, Juan, del Pulgar, Teresa Gómez, Cebrián, Arancha, Álvarez-Ayerza, Nestor, Lacal, Juan Carlos
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.06.2011; Expert Reviews Ltd; Informa Healthcare
• Subjects: biofluid; Biological markers; Biomarkers, Tumor - urine; blood; bottom-up; Cancer; cancer biomarker; Care and treatment; Genes; Humans; LC-MS/MS; Neoplasms - metabolism; Neoplasms - urine; plasma; protein depletion; Proteins; proteomics; Proteomics - methods; serum; urine
• ISSN: 1478-9450; 1744-8387
• DOI: 10.1586/epr.11.26

In the last decade, several reports have focused on the identification and characterization of proteins present in urine. In an effort to build a list of proteins of interest as biomarkers, we reviewed the largest urine proteomes and built two updated lists of proteins of interest (available as supplementary tables). The first table includes a consensus list of 443 proteins found in urine by independent laboratories and reported on the top three largest urine proteomes currently published. This consensus list of proteins could serve as biomarkers to diagnose, monitor and manage a number of diseases. Here, we focus on a reduced list of 35 proteins with potential interest as cancer biomarkers in urine following two criteria: first, proteins previously detected in urine using bottom-up proteomic experiments, and second, those suggested as cancer protein biomarkers in human plasma. In an effort to standardize the information presented and its use in future studies, here we include the updated International Protein Index (v. 3.80) and primary Swiss-Prot accession numbers, official gene symbols and recommended full names. The main variables that influence urine proteomic experiments are also discussed.