Effects of fluoride-ion-implanted titanium surface on the cytocompatibility in vitro and osseointegatation in vivo for dental implant applications

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 136; pp. 752 - 760
• Main Authors: Wang, Xue-jin, Liu, Hui-ying, Ren, Xiang, Sun, Hui-yan, Zhu, Li-ying, Ying, Xiao-xia, Hu, Shu-hai, Qiu, Ze-wen, Wang, Lang-ping, Wang, Xiao-feng, Ma, Guo-wu
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2015
• Subjects: alkaline phosphatase; Biocompatibility; Biocompatible Materials; Biomaterials; Biomedical materials; bone formation; Cell Line; coculture; colloids; Cytocompatibility; Dental implant; Dental Implants; dental prosthesis; fluorescence; fluorescent dyes; Fluoride; Fluorides; Fluorides - chemistry; Humans; in vitro studies; mineralization; Osseointegration; Plasma immersion ion implantation; Porphyromonas gingivalis; quantitative polymerase chain reaction; scanning electron microscopes; Scanning electron microscopy; staining; Surface Properties; Surgical implants; Titanium; Titanium - chemistry; Titanium base alloys; toluidine blue; Titanium; Osseointegration; Fluoride; Cytocompatibility; Dental implant; Plasma immersion ion implantation
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2015.09.039

[Display omitted] •Fluoride ion-implanted titanium (F-Ti) was prepared for dental implant application.•A co-cultured model of MG-63 and P. g was established on the modified surface.•F-Ti enhanced the attachment, proliferation, differentiation of co-cultured MG-63.•F-Ti weakens the effects of P. g exerting on the activity of MG-63.•F-Ti surface was shown to promote early osseointegration by histological study.•Both in vitro and in vivo experiments indicated the excellent biocompatibility of F-Ti. As an attractive technique for the improvement of biomaterials, Plasma immersion ion implantation (PIII) has been applied to modifying the titanium material for dental implant application. The present study investigated the cytocompatibility and early osseointegration of fluoride-ion-implanted titanium (F-Ti) surface and implants, both characterizing in their composition of titanium oxide and titanium fluoride. The cytocompatibility of F-Ti was evaluated in vitro by using scanning electron microscope, Cell Counting Kit-8 assay, alkaline phosphatase activity assay, and quantitative real-time polymerase chain reaction. The results showed that the F-Ti weakened the effects that Porphyromonas gingivalis exerted on the MG-63 cells in terms of morphology, proliferation, differentiation, and genetic expression when MG-63 cells and Porphyromonas gingivalis were co-cultured on the surface of F-Ti. Meanwhile, the osteogenic activity of F-Ti implants was assessed in vivo via evaluating the histological morphology and estimating histomorphometric parameters. The analysis of toluidine blue staining indicated that the new bone was more mature in subjects with F-Ti group, which exhibited the Haversian system, and the mean bone-implant contact value of F-Ti group was slightly higher than that of cp-Ti group (p>0.05). Fluorescence bands were wider and brighter in the F-Ti group, and the intensity of fluorochromes deposited at the sites of mineralized bone formation was significantly higher for F-Ti surfaces than for cp-Ti surfaces, within the 2nd, 3rd and 4th weeks (p<0.05). An indication is that the fluoride modified titanium can promote cytocompatibility and early osseointegration, thus providing a promising alternative for clinical use.