Solenopsis invicta virus-1A (SINV-1A): Distinct species or genotype of SINV-1?
We have cloned and sequenced a 2845 bp cDNA representing the 3′-end of either a new picorna-like virus species or genotype of Solenopsis invicta virus-1 (SINV-1). Analysis of the nucleotide sequence revealed 1 large open reading frame. The amino acid sequence of the translated open reading frame was...
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Published in | Journal of invertebrate pathology Vol. 88; no. 3; pp. 232 - 237 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier Inc
01.03.2005
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | We have cloned and sequenced a 2845
bp cDNA representing the 3′-end of either a new picorna-like virus species or genotype of
Solenopsis invicta virus-1 (SINV-1). Analysis of the nucleotide sequence revealed 1 large open reading frame. The amino acid sequence of the translated open reading frame was most identical to structural proteins of SINV-1 (97%), followed by the Kashmir bee virus (KBV, 30%), and acute bee paralysis virus (ABPV, 29%). A PCR-based survey for SINV-1 and the new species or genotype (tentatively named
S. invicta virus-1A, SINV-1A) using RNA extracts of
S. invicta collected around Gainesville, Florida, revealed a mean colony infestation rate of 25% by SINV-1 and 55% by SINV-1A. Both SINV-1 and SINV-1A were found to co-infect 17.5% of the nests surveyed. Although the data preclude definitive species or genotype assignment, there is no doubt that SINV-1A is distinct from SINV-1, identifiable, and infects
S. invicta. We provide a simple RT-PCR technique capable of discerning SINV-1 and SINV-1A infection of
S. invicta. |
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Bibliography: | http://hdl.handle.net/10113/3217 http://dx.doi.org/10.1016/j.jip.2005.02.006 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0022-2011 1096-0805 |
DOI: | 10.1016/j.jip.2005.02.006 |