Isolation and HPLC quantitation of kaurane-type diterpenes and cinnamic acid derivatives of long-term stored leaves of Mikania laevigata and Mikania glomerata

• Published in: Anais da Academia Brasileira de Ciências Vol. 85; no. 2; pp. 473 - 486
• Main Authors: Bertolucci, Suzan K V, Pereira, Ana B D, Pinto, José E B P, Oliveira, Alaíde B, Braga, Fernão C
• Format: Journal Article
• Language: English
• Published: Brazil Academia Brasileira de Ciências 01.06.2013
• Subjects: análises por CLAE-DAD; chemical markers changes; Chromatography, High Pressure Liquid; Cinnamates - isolation & purification; Diterpenes, Kaurane - isolation & purification; estocagem; HPLC-DAD analysis; marcadores químicos; Mikania - chemistry; Mikania glomerata; Mikania laevigata; MULTIDISCIPLINARY SCIENCES; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Leaves - chemistry; post-harvest; pós-colheita; storage; Time Factors; pos-colheita; HPLC-DAD analysis; Mikania glomerata; Mikania laevigata; storage; analises por CLAE-DAD; estocagem; chemical markers changes; post-harvest; marcadores quimicos
• ISSN: 0001-3765; 1678-2690; 1678-2690
• DOI: 10.1590/S0001-37652013005000029

The leaves of Mikania laevigata and Mikania glomerata are used in Brazil to treat respiratory affections, being kaurane-type diterpenes and coumarin considered as the bioactive compounds. The present study reports an investigation on the HPLC-DAD profiles and contents of coumarin (1), trans-o-coumaric (2), kaurenoic (3), benzoylgrandifloric (4) and cinnamoylgrandifloric (5) acids in dried leaves of Mikania species stored in dark room under controlled conditions. Excepting 2, the constituents were isolated and purified to be employed as reference compounds. The samples were analyzed at three monthly intervals up to 18 months for M. laevigata and 12 months for M. glomerata. trans-o-Coumaric was not detected in both, whereas 1 occurred only in M. laevigata. The concentrations of the assayed constituents did not vary significantly within the evaluated period (p < 0.05), for both species. In contrast, changes in the chromatographic profiles and spectral purity of peaks from 3, 4 and 5 were detected in samples of both Mikania stored for three months, while the coumarin profile in M. laevigata modified after six months of storage. The evaluation of chromatographic profiles based on spectral purity analyses of selected peaks was shown to be a more robust tool to access chemical stability of Mikania samples than the quantitation of chemical markers' contents.