Systematic discovery of new genes in the Saccharomyces cerevisiae genome
We used genome-wide comparative analysis of predicted protein sequences to identify many novel small genes, named smORFs for small open reading frames, within the budding yeast genome. Further analysis of 117 of these new genes showed that 84 are transcribed. We extended our analysis of one smORF co...
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Published in | Genome research Vol. 13; no. 2; pp. 264 - 271 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Cold Spring Harbor Laboratory Press
01.02.2003
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Abstract | We used genome-wide comparative analysis of predicted protein sequences to identify many novel small genes, named smORFs for small open reading frames, within the budding yeast genome. Further analysis of 117 of these new genes showed that 84 are transcribed. We extended our analysis of one smORF conserved from yeast to human. This investigation provides an updated and comprehensive annotation of the yeast genome, validates additional concepts in the study of genomes in silico, and increases the expected numbers of coding sequences in a genome with the corresponding impact on future functional genomics and proteomics studies. |
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AbstractList | We used genome-wide comparative analysis of predicted protein sequences to identify many novel small genes, named smORFs for small open reading frames, within the budding yeast genome. Further analysis of 117 of these new genes showed that 84 are transcribed. We extended our analysis of one smORF conserved from yeast to human. This investigation provides an updated and comprehensive annotation of the yeast genome, validates additional concepts in the study of genomes in silico, and increases the expected numbers of coding sequences in a genome with the corresponding impact on future functional genomics and proteomics studies. We used genome-wide comparative analysis of predicted protein sequences to identify many novel small genes, named smORFs for sm all o pen r eading f rames, within the budding yeast genome. Further analysis of 117 of these new genes showed that 84 are transcribed. We extended our analysis of one smORF conserved from yeast to human. This investigation provides an updated and comprehensive annotation of the yeast genome, validates additional concepts in the study of genomes in silico, and increases the expected numbers of coding sequences in a genome with the corresponding impact on future functional genomics and proteomics studies. |
Author | Zeng, Qiandong Morales, Arturo J Cottarel, Guillaume Hogan, Sarah Kessler, Marco M Cook, Robin |
AuthorAffiliation | Genome Therapeutics Corporation, Waltham, Massachusetts 02453, USA |
AuthorAffiliation_xml | – name: Genome Therapeutics Corporation, Waltham, Massachusetts 02453, USA |
Author_xml | – sequence: 1 givenname: Marco M surname: Kessler fullname: Kessler, Marco M organization: Genome Therapeutics Corporation, Waltham, Massachusetts 02453, USA – sequence: 2 givenname: Qiandong surname: Zeng fullname: Zeng, Qiandong – sequence: 3 givenname: Sarah surname: Hogan fullname: Hogan, Sarah – sequence: 4 givenname: Robin surname: Cook fullname: Cook, Robin – sequence: 5 givenname: Arturo J surname: Morales fullname: Morales, Arturo J – sequence: 6 givenname: Guillaume surname: Cottarel fullname: Cottarel, Guillaume |
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Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Undefined-3 E-MAIL arturo.morales@genomecorp.com; FAX (781) 398-2476. Article and publication are at http://www.genome.org/cgi/doi/10.1101/gr.232903. Corresponding author. |
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SubjectTerms | Amino Acid Sequence - genetics Animals Caenorhabditis elegans Proteins - genetics Cattle Chromosome Mapping - methods Chromosomes, Fungal - genetics Conserved Sequence - genetics Drosophila Proteins - genetics Genes, Fungal - genetics Genome, Fungal Humans Letters Mice Molecular Sequence Data Open Reading Frames - genetics Proteins - genetics Saccharomyces cerevisiae - genetics Sequence Homology, Amino Acid |
Title | Systematic discovery of new genes in the Saccharomyces cerevisiae genome |
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