Systematic discovery of new genes in the Saccharomyces cerevisiae genome

• Published in: Genome research Vol. 13; no. 2; pp. 264 - 271
• Main Authors: Kessler, Marco M, Zeng, Qiandong, Hogan, Sarah, Cook, Robin, Morales, Arturo J, Cottarel, Guillaume
• Format: Journal Article
• Language: English
• Published: United States Cold Spring Harbor Laboratory Press 01.02.2003
• Subjects: Amino Acid Sequence - genetics; Animals; Caenorhabditis elegans Proteins - genetics; Cattle; Chromosome Mapping - methods; Chromosomes, Fungal - genetics; Conserved Sequence - genetics; Drosophila Proteins - genetics; Genes, Fungal - genetics; Genome, Fungal; Humans; Letters; Mice; Molecular Sequence Data; Open Reading Frames - genetics; Proteins - genetics; Saccharomyces cerevisiae - genetics; Sequence Homology, Amino Acid
• ISSN: 1088-9051; 1549-5469
• DOI: 10.1101/gr.232903

We used genome-wide comparative analysis of predicted protein sequences to identify many novel small genes, named smORFs for small open reading frames, within the budding yeast genome. Further analysis of 117 of these new genes showed that 84 are transcribed. We extended our analysis of one smORF conserved from yeast to human. This investigation provides an updated and comprehensive annotation of the yeast genome, validates additional concepts in the study of genomes in silico, and increases the expected numbers of coding sequences in a genome with the corresponding impact on future functional genomics and proteomics studies.