Increased elasticity of melanoma cells after low-LET proton beam due to actin cytoskeleton rearrangements

• Published in: Scientific reports Vol. 9; no. 1; p. 7008
• Main Authors: Jasińska-Konior, Katarzyna, Wiecheć, Olga, Sarna, Michał, Panek, Agnieszka, Swakoń, Jan, Michalik, Marta, Urbańska, Krystyna, Elas, Martyna
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 07.05.2019; Nature Publishing Group UK
• Subjects: Actin; Actin Cytoskeleton - metabolism; Actin Cytoskeleton - radiation effects; Angiogenesis; Cell Line, Tumor; Cell migration; Cell Movement - radiation effects; Cell Proliferation - radiation effects; Cell Survival - radiation effects; Cytoskeleton; DNA repair; Elasticity - radiation effects; Gene Expression Regulation, Neoplastic - radiation effects; Humans; Melanoma; Melanoma - metabolism; Melanoma - radiotherapy; Melanoma, Cutaneous Malignant; Proton Therapy - methods; Skin Neoplasms - metabolism; Skin Neoplasms - radiotherapy; Up-Regulation; Uveal Neoplasms - metabolism; Uveal Neoplasms - radiotherapy; Vimentin; Vimentin - metabolism
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-43453-7

Cellular response to non-lethal radiation stress include perturbations in DNA repair, angiogenesis, migration, and adhesion, among others. Low-LET proton beam radiation has been shown to induce somewhat different biological response than photon radiation. For example, we have shown that non-lethal doses of proton beam radiation inhibited migration of cells and that this effect persisted long-term. Here, we have examined cellular elasticity and actin cytoskeleton organization in BLM cutaneous melanoma and Mel270 uveal melanoma cells. Proton beam radiation increased cellular elasticity to a greater extent than X-rays and both types of radiation induced changes in actin cytoskeleton organization. Vimentin level increased in BLM cells after both types of radiation. Our data show that cell elasticity increased substantially after low-LET proton beam and persisted long after radiation. This may have significant consequences for the migratory properties of melanoma cells, as well as for the cell susceptibility to therapy.