Expression of enhancer binding factors associated with various cell types of lung cancer

• Published in: Biochemical and biophysical research communications Vol. 180; no. 2; pp. 1064 - 1069
• Main Authors: Wong, Yuk-Chor, Bernal, Samuel D.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 31.10.1991; Elsevier
• Subjects: Adenocarcinoma - genetics; Base Sequence; Binding Sites; Biological and medical sciences; Carcinoma - genetics; Cell Line; DNA-Binding Proteins - genetics; Enhancer Elements, Genetic; Host-tumor relations. Immunology. Biological markers; Humans; Lung Neoplasms - genetics; Medical sciences; Molecular Sequence Data; Nuclear Proteins - metabolism; Oligodeoxyribonucleotides; Proto-Oncogene Proteins c-jun - genetics; Transcription Factors - genetics; Transcription Factors - metabolism; Tumors; Human; Cell culture; Enhancer sequence; Carcinoma; Type specificity; Lung; Tumor; Molecular probe; Gene expression
• DOI: 10.1016/S0006-291X(05)81174-X
• ISSN: 0006-291X

Differential expression of nuclear factors binding specifically to AP-1, AP-2, AP-3, and NF-I/CTF enhancer elements was found in the various cell types of human lung carcinoma cells. Adenocarcinoma and squamous carcinoma cells seemed to express higher levels of these factors than large-cell carcinoma and small-cell carcinoma cells. Among small-cell carcinoma cells, variant small-cell carcinoma cells which presumably are closer than classical small-cell carcinoma cells to non-small-cell carcinoma cells in terms of differentiation characteristics also expressed higher levels of these factors. Furthermore, nuclear extracts from the various cell types of carcinoma cells were found to produce different patterns of electrophoretic mobility shift even with the same enhancer element, suggesting the presence of multiple species of specific enhancer-binding activities in these cells. Thus, these enhancer elements may be used as probes for cell-type specificity in the study of differentiation of lung carcinomas.