Expression of enhancer binding factors associated with various cell types of lung cancer

Differential expression of nuclear factors binding specifically to AP-1, AP-2, AP-3, and NF-I/CTF enhancer elements was found in the various cell types of human lung carcinoma cells. Adenocarcinoma and squamous carcinoma cells seemed to express higher levels of these factors than large-cell carcinom...

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Published inBiochemical and biophysical research communications Vol. 180; no. 2; pp. 1064 - 1069
Main Authors Wong, Yuk-Chor, Bernal, Samuel D.
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier Inc 31.10.1991
Elsevier
Subjects
Adenocarcinoma - genetics
Base Sequence
Binding Sites
Biological and medical sciences
Carcinoma - genetics
Cell Line
DNA-Binding Proteins - genetics
Enhancer Elements, Genetic
Host-tumor relations. Immunology. Biological markers
Humans
Lung Neoplasms - genetics
Medical sciences
Molecular Sequence Data
Nuclear Proteins - metabolism
Oligodeoxyribonucleotides
Proto-Oncogene Proteins c-jun - genetics
Transcription Factors - genetics
Transcription Factors - metabolism
Tumors
Human
Cell culture
Enhancer sequence
Carcinoma
Type specificity
Lung
Tumor
Molecular probe
Gene expression
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Summary:Differential expression of nuclear factors binding specifically to AP-1, AP-2, AP-3, and NF-I/CTF enhancer elements was found in the various cell types of human lung carcinoma cells. Adenocarcinoma and squamous carcinoma cells seemed to express higher levels of these factors than large-cell carcinoma and small-cell carcinoma cells. Among small-cell carcinoma cells, variant small-cell carcinoma cells which presumably are closer than classical small-cell carcinoma cells to non-small-cell carcinoma cells in terms of differentiation characteristics also expressed higher levels of these factors. Furthermore, nuclear extracts from the various cell types of carcinoma cells were found to produce different patterns of electrophoretic mobility shift even with the same enhancer element, suggesting the presence of multiple species of specific enhancer-binding activities in these cells. Thus, these enhancer elements may be used as probes for cell-type specificity in the study of differentiation of lung carcinomas.
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ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(05)81174-X