Structural characterization and immunomodulating activity of polysaccharide from Dendrobium officinale

• Published in: International journal of biological macromolecules Vol. 83; pp. 34 - 41
• Main Authors: He, Tao-Bin, Huang, Yan-Ping, Yang, Liu, Liu, Ti-Ti, Gong, Wan-Ying, Wang, Xuan-Jun, Sheng, Jun, Hu, Jiang-Miao
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2016
• Subjects: 2,2-diphenyl-1-picrylhydrazyl; antioxidant activity; Antioxidants - chemistry; Antioxidants - pharmacology; Biphenyl Compounds - pharmacology; Cells, Cultured; Dendrobium - chemistry; Dendrobium officinale; Fourier transform infrared spectroscopy; glucomannans; glucose; Humans; Immunomodulation; Immunomodulation - drug effects; immunomodulators; interleukin-10; Interleukin-10 - metabolism; interleukin-1beta; Interleukin-1beta - metabolism; mannose; Mannose - metabolism; molecular weight; nuclear magnetic resonance spectroscopy; Picrates - pharmacology; Plant Extracts - chemistry; Plant Extracts - pharmacology; Polysaccharide; Polysaccharides - chemistry; Polysaccharides - pharmacology; secretion; signal transduction; Spectroscopy, Fourier Transform Infrared - methods; tumor necrosis factor-alpha; Tumor Necrosis Factor-alpha - metabolism; Immunomodulation; Dendrobium officinale; Polysaccharide
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2015.11.038

•A neutral heteropolysaccharide was purified from dried stem Dendrobium officinale.•The structure of DOP-1-1 was partially elucidated.•The antioxidant and immune activities of DOP-1-1 was evaluated.•DOP-1-1 exhibited significant immune-modulating effect and the immune mechanism of DOP-1-1 was through upregulating NF-кB and ERK1/2. A neutral heteropolysaccharide (DOP-1-1) consisted by mannose and glucose (5.9:1) with an average molecular weight at about 1.78×105Da was purified from Dendrobium officinale. Based on Fourier transform infrared spectrum (FT-IR) and nuclear magnetic resonance (NMR) spectra, it suggested that partial structure of DOP-1-1 is an O-acetylated glucomannan with β-d configuration in pyranose sugar forms. The immunomodulatory activity of DOP-1-1 was evaluated by secretion level of cytokine (interleukin (IL)-1β and IL-10) and tumor necrosis factor (TNF)-α in vitro. Our results suggested that DOP-1-1 could stimulate cytokine production (TNF-α, IL-1β) in cells. These findings demonstrated that the purified polysaccharide from D. officinale presented significant immune-modulating activities. Furthermore, by Western-blot we can found that the signaling pathways of DOP-1-1 induced immune activities involving ERK1/2 and NF-кB. As to antioxidant activity, DOP-1-1 hadn’t showed remarkable scavenging capacity of 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) in contrast with other studies of polysaccharides from D. officinale.