On the Site of Sulfation in the Chondrocyte

As observed autoradiographically in the cartilage of embryonic rats, radiosulfate is bound and concentrated only in vesicles of the juxtanuclear Golgi apparatus of secreting chondrocytes within 3 minutes of its presentation. From this area, vacuoles migrate peripherally and lodge in the subcortex; t...

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Bibliographic Details
Published inThe Journal of cell biology Vol. 21; no. 3; pp. 353 - 366
Main Authors Godman, Gabriel C., Lane, Nathan
Format Journal Article
LanguageEnglish
Published United States Rockefeller Institute Press 01.06.1964
The Rockefeller University Press
Subjects
Autoradiography
Biological Transport
Cartilage
Cells
Chondrocytes
Cytoplasm
Embryo, Mammalian
Embryo, Nonmammalian
Golgi Apparatus
Metabolism
Old Medline
Polysaccharides
Radio observatories
Rats
Sulfates
Sulfation
Vacuoles
GOLGI APPARATUS
SULFATES
EMBRYO
METABOLISM
EXPERIMENTAL LAB STUDY
RATS
RADIOAUTOGRAPHY
CARTILAGE
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Summary:As observed autoradiographically in the cartilage of embryonic rats, radiosulfate is bound and concentrated only in vesicles of the juxtanuclear Golgi apparatus of secreting chondrocytes within 3 minutes of its presentation. From this area, vacuoles migrate peripherally and lodge in the subcortex; their sulfated contents are thence discharged via stomata to the extracellular matrix. The label, apparently often associated with microvesicles at 10 and 20 minutes, is subsequently localized in the dense contents of the larger vacuoles. Bound radiosulfate is not detectable in other organelles. It is concluded that the vesicular component of the Golgi apparatus is the actual site of sulfation. Intracellular hyaluronidasesensitive metachromatic granules are found chiefly at the cell periphery or mantle, rarely juxtanuclear in the main Golgi zone.
Bibliography:ObjectType-Article-1
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content type line 23
Dr. Lane's present address is Laboratory of Surgical Pathology, College of Physicians and Surgeons
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.21.3.353