Curcumin hampers the antitumor effect of vinblastine via the inhibition of microtubule dynamics and mitochondrial membrane potential in HeLa cervical cancer cells

• Published in: Phytomedicine (Stuttgart) Vol. 23; no. 7; pp. 705 - 713
• Main Authors: Lee, Jae-Wook, Park, Sojin, Kim, Sun Yeou, Um, Sung Hee, Moon, Eun-Yi
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 15.06.2016; Urban & Fischer Verlag
• Subjects: Animals; Antineoplastic Agents, Phytogenic - pharmacology; Apoptosis; Apoptosis - drug effects; Care and treatment; Caspase 3 - metabolism; Cell Survival - drug effects; Cervical cancer; Curcumin; Curcumin - pharmacology; Drug Synergism; Female; Health aspects; HeLa Cells; Humans; Male; Medicine, Botanic; Medicine, Herbal; Membrane Potential, Mitochondrial - drug effects; Mice, Inbred C57BL; Microtubule; Microtubules - drug effects; Mitochondrial membrane potential; Reactive Oxygen Species - metabolism; ROS, Antitumor activity; Tetrazolium Salts; Thiazoles; Turmeric; Vinblastine; Vinblastine - pharmacology; South Korea; Vinblastine; DAPI; MTT; MMP; NAC; WST-8; ROS; PI; DCF-DA; Curcumin; Microtubule; ROS, Antitumor activity; Mitochondrial membrane potential; JC-1
• ISSN: 0944-7113; 1618-095X
• DOI: 10.1016/j.phymed.2016.03.011

Curcumin, a major component of curry powder, which is a natural polyphenol product extracted from rhizoma curcumae longae, interacts with a specific binding site on microtubules. Vinblastine is an antitumor drug that induces microtubule depolymerization. We investigated whether curcumin influences the antitumor effect of vinblastine in HeLa human cervical cancer cells. Changes in microtubule filaments were visualized by immuno-staining. Cell death was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) or water-soluble tetrazolium(WST) assay. Apoptotic cell formation was assessed by flow cytometry after staining cells with propidium iodide(PI) and/or Annexin V or with 6-diamidino-2-phenylindole(DAPI). Reactive oxygen species(ROS) were also measured by flow cytometry using dichloro-dihydro-fluorescein diacetate(DCF-DA). JC-1 was used to determine mitochondrial membrane potential (MMP). When cells were pretreated with curcumin, microtubule filaments were disordered. Vinblastine-induced microtubule depolymerization and cell death were reduced in HeLa human cervical cancer cells pretreated with curcumin compared to the control. The decrease in cell death was much greater in cells pretreated with curcumin compared to cotreatment or post-treatment. DNA condensation by vinblastine was also decreased in curcumin-pretreated cells. Curcumin reduced ROS production by vinblastine. However, no changes in vinblastine-mediated microtubule depolymerization were detected upon N-acetylcysteine(NAC) treatment. In contrast, vinblastine-induced MMP collapse was inhibited by pretreatment with curcumin or NAC. These findings suggest that vinblastine-induced tumor cell death might be inhibited by curcumin via ROS-independent microtubule dynamics and ROS-dependent MMP collapse. It also suggests that microtubule dynamics could be necessary for the optimal antitumor activity of vinblastine. Our results suggest that patients treated with vinblastine should not consume curcumin. [Display omitted]