Mixed epithelial and stromal tumor of the kidney lacks the genetic alterations of cellular congenital mesoblastic nephroma

• Published in: Human pathology Vol. 32; no. 5; pp. 513 - 520
• Main Authors: Pierson, Christopher R., Schober, Megan S., Wallis, Tracie, Sarkar, Fazlul H., Sorensen, Poul H.B., Eble, John N., Srigley, John R., Jones, Edward C., Grignon, David J., Adsay, Volkan
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.05.2001; Elsevier
• Subjects: Adult; Aged; Biological and medical sciences; biphasic; Chromosomes, Human, Pair 11; Chromosomes, Human, Pair 17; Chromosomes, Human, Pair 8; DNA-Binding Proteins - genetics; Epithelial Cells - pathology; ETS Translocation Variant 6 Protein; ETV6-NTRK3 gene fusion; Female; Humans; In Situ Hybridization, Fluorescence; Kidney Neoplasms - genetics; Kidney Neoplasms - pathology; Kidneys; Medical sciences; Menopause; mesoblastic nephroma; Middle Aged; mixed tumor; Nephrology. Urinary tract diseases; Nephroma, Mesoblastic - congenital; Nephroma, Mesoblastic - genetics; Oncogene Proteins, Fusion; Ploidies; polyploidy; Proto-Oncogene Proteins c-ets; Receptor, trkC - genetics; Repressor Proteins; Reverse Transcriptase Polymerase Chain Reaction; reverse-transcription polymerase chain reaction; Stromal Cells - pathology; Transcription Factors - genetics; Translocation, Genetic; Tumors of the urinary system; mesoblastic nephroma; ETV6-NTRK3 gene fusion; polyploidy; RT-PCR; mixed tumor; FISH; IGF; biphasic; reverse-transcription polymerase chain reaction; Human; Kidney disease; Chromosomal aberration; Immunohistochemistry; Urinary system disease; Mesoblastic nephroma; Kidney; Pathology; Fluorescence in situ hybridization; Adult; Epithelial cell; Female; Stromal cell; Diagnosis; Benign neoplasm; Molecular biology; Comparative study
• ISSN: 0046-8177; 1532-8392
• DOI: 10.1053/hupa.2001.24323

Mixed epithelial and stromal tumor of the kidney is a recently recognized neoplasm that occurs almost exclusively in perimenopausal women. Because it frequently contains areas of smooth muscle in which epithelial structures are embedded, some have concluded that it is the adult form of congenital mesoblastic nephroma. Others have concluded that the morphology and epidemiology of mixed epithelial and stromal tumor indicate that it is unrelated to congenital mesoblastic nephroma. Although the genetic alterations of mixed epithelial and stromal tumor have not been previously elucidated, much is known about the genetic alterations of cellular congenital mesoblastic nephroma. The present study was undertaken to determine if mixed epithelial and stromal tumors have any of the genetic alterations recognized as typical of cellular congenital mesoblastic nephroma. RNA extraction was performed on formalin-fixed, paraffin-embedded tissue from 7 mixed epithelial and stromal tumors followed by reverse-transcription polymerase chain reaction to detect the ETV6-NTRK3 gene fusion. Fluorescent in situ hybridization with centromere-specific probes for chromosomes 8, 11, and 17 was performed to evaluate polyploidy of these chromosomes in 11 cases of mixed epithelial and stromal tumor. None of the mixed epithelial and stromal tumors showed any of these genetic alterations. We conclude that mixed epithelial and stromal tumor of the kidney lacks the genetic alterations typical of cellular congenital mesoblastic nephroma, is unrelated to it, and the appellation “adult mesoblastic nephroma” should not be used for these tumors. HUM PATHOL 32:513-520. Copyright © 2001 by W.B. Saunders Company