Cytotoxic Activities against Breast Cancer Cells of Local Justicia gendarussa Crude Extracts

Justicia gendarussa methanolic leaf extracts from five different locations in the Southern region of Peninsular Malaysia and two flavonoids, kaempferol and naringenin, were tested for cytotoxic activity. Kaempferol and naringenin were two flavonoids detected in leaf extracts using gas chromatography...

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Published inEvidence-based complementary and alternative medicine Vol. 2014; no. 2014; pp. 1 - 12
Main Authors Jamil, Shajarahtunnur, Abd Samad, Azman, Mohd Bohari, Siti Pauliena, Ayob, Zahidah
Format Journal Article
LanguageEnglish
Published Cairo, Egypt Hindawi Publishing Corporation 01.01.2014
Hindawi Limited
Subjects
Alternative medicine
Apoptosis
Biodiversity
Biotechnology
Breast cancer
Cancer therapies
Cytotoxicity
Flame ionization
Flavonoids
Gas chromatography
Herbal medicine
Ionization
Kaempferol
Laboratories
Leaves
Lung cancer
Medical research
Metastasis
Naringenin
Stem cells
Tumor cell lines
Malaysia
Selangor Malaysia
United States > US
Indonesia
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Summary:Justicia gendarussa methanolic leaf extracts from five different locations in the Southern region of Peninsular Malaysia and two flavonoids, kaempferol and naringenin, were tested for cytotoxic activity. Kaempferol and naringenin were two flavonoids detected in leaf extracts using gas chromatography-flame ionization detection (GC-FID). The results indicated that highest concentrations of kaempferol and naringenin were detected in leaves extracted from Mersing with 1591.80 mg/kg and 444.35 mg/kg, respectively. Positive correlations were observed between kaempferol and naringenin concentrations in all leaf extracts analysed with the Pearson method. The effects of kaempferol and naringenin from leaf extracts were examined on breast cancer cell lines (MDA-MB-231 and MDA-MB-468) using MTT assay. Leaf extract from Mersing showed high cytotoxicity against MDA-MB-468 and MDA-MB-231 with IC50 values of 23 μg/mL and 40 μg/mL, respectively, compared to other leaf extracts. Kaempferol possessed high cytotoxicity against MDA-MB-468 and MDA-MB-231 with IC50 values of 23 μg/mL and 34 μg/mL, respectively. These findings suggest that the presence of kaempferol in Mersing leaf extract contributed to high cytotoxicity of both MDA-MB-231 and MDA-MB-468 cancer cell lines.
Bibliography:ObjectType-Article-1
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Academic Editor: Wagner Vilegas
ISSN:1741-427X
1741-4288
DOI:10.1155/2014/732980