Quantitative deviating effects of maple syrup extract supplementation on the hepatic gene expression of mice fed a high‐fat diet

• Published in: Molecular nutrition & food research Vol. 61; no. 2; pp. np - n/a
• Main Authors: Kamei, Asuka, Watanabe, Yuki, Shinozaki, Fumika, Yasuoka, Akihito, Shimada, Kousuke, Kondo, Kaori, Ishijima, Tomoko, Toyoda, Tsudoi, Arai, Soichi, Kondo, Takashi, Abe, Keiko
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.02.2017
• Subjects: Acer - chemistry; Amino acids; Animals; Cholesterol; Diet; Diet, High-Fat; Dietary Sugars - pharmacology; Dietary Supplements; Endoplasmic reticulum; Enrichment; Fatty acids; Fatty Acids - metabolism; Folic acid; Gene expression; Gene expression analysis; Gene Expression Regulation; Gluconeogenesis; Glycolysis; High fat diet; Ingestion; Lipid metabolism; Liver; Liver - drug effects; Liver - physiology; Male; Maple syrup; Metabolism; Mice; Mice, Inbred C57BL; Polyphenol; Polyphenols; Rodents; Syrup; Syrups; Tricarboxylic acid cycle; Gene expression analysis; Polyphenol; High-fat diet; Liver; Maple syrup
• ISSN: 1613-4125; 1613-4133
• DOI: 10.1002/mnfr.201600477

Scope Maple syrup contains various polyphenols and we investigated the effects of a polyphenol‐rich maple syrup extract (MSXH) on the physiology of mice fed a high‐fat diet (HFD). Methods and results The mice fed a low‐fat diet (LFD), an HFD, or an HFD supplemented with 0.02% (002MSXH) or 0.05% MSXH (005MSXH) for 4 weeks. Global gene expression analysis of the liver was performed, and the differentially expressed genes were classified into three expression patterns; pattern A (LFD < HFD > 002MSXH = 005MSXH, LFD > HFD < 002MSXH = 005MSXH), pattern B (LFD < HFD = 002MSXH > 005MSXH, LFD > HFD = 002MSXH < 005MSXH), and pattern C (LFD < HFD > 002MSXH < 005MSXH, LFD > HFD < 002MSXH > 005MSXH). Pattern A was enriched in glycolysis, fatty acid metabolism, and folate metabolism. Pattern B was enriched in tricarboxylic acid cycle while pattern C was enriched in gluconeogenesis, cholesterol metabolism, amino acid metabolism, and endoplasmic reticulum stress‐related event. Conclusion Our study suggested that the effects of MSXH ingestion showed (i) dose‐dependent pattern involved in energy metabolisms and (ii) reversely pattern involved in stress responses. Ingestion of a polyphenol‐rich maple syrup extract (MSXH, containing 15.02% w/w polyphenols) has effects on the hepatic gene expression of mice fed a high‐fat diet. Global gene expression analysis of the liver is performed, and the differentially expressed genes are classified into three expression patterns. Patterns A and B in this figure involve genes working in energy metabolisms and pattern C involves genes in stress responses.