Ammonia-oxidizing Archaea in laboratory-scale activated sludge systems for wastewater of low- or high-ammonium concentration

• Published in: Animal science journal Vol. 83; no. 7; pp. 571 - 576
• Main Authors: OISHI, Ryu, HIROOKA, Kayako, OTAWA, Kenichi, TADA, Chika, NAKAI, Yutaka
• Format: Journal Article
• Language: English
• Published: Melbourne, Australia Blackwell Publishing Asia 01.07.2012; Blackwell Publishing Ltd
• Subjects: activated sludge; Ammonia; Ammonia - analysis; Ammonia - metabolism; ammonia monooxygenase (AMO); ammonia-oxidizing Archaea; Archaea; Archaea - enzymology; Archaea - genetics; Archaea - metabolism; Bacteria; denaturing gel gradient electrophoresis (DGGE); Denaturing Gradient Gel Electrophoresis; Oxidation-Reduction; Oxidoreductases - genetics; Oxidoreductases - metabolism; Sewage - chemistry; Sewage - microbiology; Sludge; Waste Water - chemistry; Waste Water - microbiology; Water treatment
• ISSN: 1344-3941; 1740-0929
• DOI: 10.1111/j.1740-0929.2011.00995.x

ABSTRACT Ammonia‐oxidizing bacteria (AOB) is known as ammonia‐oxidizer in wastewater treatment systems. However, ammonia‐oxidizing Archaea (AOA) is found from various environments, including wastewater treatment systems. In this study, to investigate the relationships between AOA population and ammonia concentration, AOA was monitored in two laboratory‐scale reactors treating artificial wastewater of different ammonium concentrations by denaturing gradient gel electrophoresis targeting ammonia monooxygenase genes. At day 60 of the operation, AOA populations dominant in each reactor differed, suggesting the importance of influent ammonia concentration in dominant AOA selection.