The hemolymph of Biomphalaria snail vectors of schistosomiasis supports a diverse microbiome

• Published in: Environmental microbiology Vol. 22; no. 12; pp. 5450 - 5466
• Main Authors: Chevalier, Frédéric D., Diaz, Robbie, McDew‐White, Marina, Anderson, Timothy J. C., Le Clec'h, Winka
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.12.2020; Wiley Subscription Services, Inc
• Subjects: Animals; Aquatic insects; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Biology; Biomphalaria; Biomphalaria - classification; Biomphalaria - microbiology; Disease Vectors; Diseases; DNA; Gastropoda; Hemolymph; Hemolymph - microbiology; Host Specificity; Insects; Microbiomes; Microbiota; Microorganisms; Mollusks; Mosquitoes; Parasites; Populations; RNA, Ribosomal, 16S - genetics; Schistosoma mansoni - physiology; Schistosomiasis; Schistosomiasis - transmission; Snails; Vectors
• ISSN: 1462-2912; 1462-2920; 1462-2920
• DOI: 10.1111/1462-2920.15303

Summary The microbiome ‐ the microorganism community that is found on or within an organism's body ‐ is increasingly recognized to shape many aspects of its host biology and is a key determinant of health and disease. Microbiomes modulate the capacity of insect disease vectors (mosquitoes, tsetse flies, sandflies) to transmit parasites and disease. We investigate the diversity and abundance of microorganisms within the hemolymph (i.e. blood) of Biomphalaria snails, the intermediate host for Schistosoma mansoni, using Illumina MiSeq sequencing of the bacterial 16S V4 rDNA. We sampled hemolymph from five snails from six different laboratory populations of B. glabrata and one population of B. alexandrina. We observed 279.84 ± 0.79 amplicon sequence variants per snail. There were significant differences in microbiome composition at the level of individual snails, snail populations and species. Snail microbiomes were dominated by Proteobacteria and Bacteroidetes while water microbiomes from snail tank were dominated by Actinobacteria. We investigated the absolute bacterial load using qPCR: hemolymph samples contained 2784 ± 339 bacteria/μl. We speculate that the microbiome may represent a critical, but unexplored intermediary in the snail–schistosome interaction as hemolymph is in very close contact with the parasite at each step of its development.